300234 VO Biochemical and biophysical characterization and validation approaches (2016S)
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Details
Sprache: Englisch
Prüfungstermine
Lehrende
- Ivan Yudushkin
- Dieter Blaas
- Gang Dong
- Markus Hartl
- Thomas Leonard
- Arthur Sedivy
- Dea Slade
- Bojan Zagrovic
Termine
Ort: SR/STB,Viennabiocenter 5, 1030 Wien.
Mandatory Student Orientation Meeting (Vorbesprechung):
17.03.2016, 15:00-16:00 (VBC5 seminar room #1.116)
dates: 5 - 13 April 2016, 09:00-10:30; 10:45-12:15 Uhr
05.04, 10:45-12:15 (Zagrovic) Math primer
06.04, 09:00-10:30 (Sedivy) Absorption spectroscopy
06.04, 10:45-12:15 (Yudushkin) Fluorescence spectroscopy
07.04, 09:00-10:30 (Sedivy) Applications of absorption and fluorescence spectroscopy
07.04, 10:45-12:15 (Hartl) Introduction to mass spectrometry
08.04, 09:00-10:30 (Slade) Protein expression and purification
08.04, 10:45-12:15 (Leonard) From protein properties to purification
11.04, 09:00-10:30 (Stolt-Bergner) Integral membrane proteins and protein complexes
11.04, 10:45-12:15 (Dong) Introduction to X-ray crystallography, SLS and SAXS
12.04, 09:00-10:30 (Yudushkin) Introduction to light microscopy
12.04, 10:45-12:15 (Blaas) Introduction to electron microscopy
13.04, 09:00-10:30 (Yudushkin) Imaging methods for detection of molecular proximity
13.04, 10:45-12:15 (Molodtsov) Force spectroscopy
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
This theoretical course will start with introductory lectures on the basic concepts in structural biology and cover modern approaches and fundamentals of the methods used in recombinant protein expression, purification, biophysical characterization and quality assessment of macromolecular samples as well as cell biophysics approaches. The covered topics include static and dynamic light scattering, fundamentals of X-ray crystallography, absorption and fluorescence spectroscopy techniques (CD, FRET, FLIM, FC(C)S, fluorescence anisotropy, etc), mass spectroscopy and imaging methods (light and electron microscopy).
Art der Leistungskontrolle und erlaubte Hilfsmittel
Verpflichtende Anwesenheit. Die Beurteilung basiert auf folgenden Teilleistungen: Aktive Teilnahme und Ergebnis der Versuche, schriftliches Protokoll, theoretisches Wissen und Abschlussprüfung (die prozentuelle Aufteilung der Teilleistungen wird vom Kursleiter bekanntgegeben). Jede der Teilleistungen muss positiv sein.
Mindestanforderungen und Beurteilungsmaßstab
Provide a comprehensive overview of strategies and methods for protein expression and purification; enable students to choose a better strategy for a given protein
Highlight the utility of different methods of protein characterization for understanding its structure and function using specific examples
Explain theoretical background and instrumentation used for protein characterization in amount sufficient for critical interpretation of original research results
Enable students to choose an adequate method for a specific question
Highlight the utility of different methods of protein characterization for understanding its structure and function using specific examples
Explain theoretical background and instrumentation used for protein characterization in amount sufficient for critical interpretation of original research results
Enable students to choose an adequate method for a specific question
Prüfungsstoff
Literatur
Zuordnung im Vorlesungsverzeichnis
PhD MB 2, MMB I-2, MMB II-2, M-WZB, MGE III-1,MMEI III, MMB W-2
Letzte Änderung: Mo 07.09.2020 15:43