300417 VO Methoden in der Zellbiologie (2011S)
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Vorbesprechung 7. März, 2011, 14:00 Uhr
BZB/SR 3.Stock/ Dr. Bohr-Gasse 9, 1030 Wien.
Block: 2.5. bis 7.6.2011 jew. Montag und Mittwoch 13.00- 15.00 Uhr!!!
Time of lectures changed to 1:00 to 3:00 pm (instead of 2-4:00 pm)May 2:Cell culture, protein expression and purification :Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems - Andreas BrachnerMay 4: Protein-Protein, Protein DNA interactions: In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays - Andreas BrachnerMay 9: Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics - Josef GotzmannMay 11: Light Microscopy: Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET - Josef GotzmannMay 16: Dynamics and Spatial Organisation of the Genome, Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping of gene territories - Alwin KöhlerMay 18: Ananlyses of cell cycle and Tumorbiology Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization
EMT, telomeres / DNA damage, transgenic and knock out tumor models - Andreas Brachner, Roland FoisnerMay 23: Proteomics: Protein-identification (LC-MS/MS, mass spec, bio-informatics - Christopher GernerMay 25: Techniques in Lipid Biochemistry - Sascha MartensMay 30: X-crystallography Protein purification, Crystallisation, X-ray analyses, structure determination - Gang Dong
BZB/SR 3.Stock/ Dr. Bohr-Gasse 9, 1030 Wien.
Block: 2.5. bis 7.6.2011 jew. Montag und Mittwoch 13.00- 15.00 Uhr!!!
Time of lectures changed to 1:00 to 3:00 pm (instead of 2-4:00 pm)May 2:Cell culture, protein expression and purification :Primary cultures, organtypical cultures, differentiation models, eukaryotic expression systems, vector systems - Andreas BrachnerMay 4: Protein-Protein, Protein DNA interactions: In vitro binding assays, affinity (Scatchard), In situ analyses (IP), in vivo analyses, FRET, yeast two hybrid Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid Reporter Gene Assays - Andreas BrachnerMay 9: Gene Silencing techniques RNA-Interferenz; Antisense, morpholino, epigenetics - Josef GotzmannMay 11: Light Microscopy: Immunofluorescence, wide field, confocal, live cell imaging, FRAP, FRET - Josef GotzmannMay 16: Dynamics and Spatial Organisation of the Genome, Chromosome Conformation Capture techniques, GFP-tagging of chromosomes, High-resolution mapping of gene territories - Alwin KöhlerMay 18: Ananlyses of cell cycle and Tumorbiology Cell Cycle Stages (FACS, Elutriation, BrdU incorporation, proliferation marker, synchronization
EMT, telomeres / DNA damage, transgenic and knock out tumor models - Andreas Brachner, Roland FoisnerMay 23: Proteomics: Protein-identification (LC-MS/MS, mass spec, bio-informatics - Christopher GernerMay 25: Techniques in Lipid Biochemistry - Sascha MartensMay 30: X-crystallography Protein purification, Crystallisation, X-ray analyses, structure determination - Gang Dong
Details
Sprache: Englisch
Prüfungstermine
Lehrende
- Roland Foisner
- Andreas Brachner
- Gang Dong
- Christopher Gerner
- Josef Gotzmann
- Alwin Köhler
- Sascha Martens
Termine
Zur Zeit sind keine Termine bekannt.
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Art der Leistungskontrolle und erlaubte Hilfsmittel
Mindestanforderungen und Beurteilungsmaßstab
Prüfungsstoff
Literatur
Zuordnung im Vorlesungsverzeichnis
MMB III-2, MMB IV-2, MMB W-2, M202, M210
Letzte Änderung: Mo 07.09.2020 15:43
Studiums erhaltenen theoretischen und praktischen Kenntnissen aufbaut. Die Inhalte werden jährlich adaptiert um neue Entwicklungen einzubauen. Wesentliche in der Vorlesung enthaltenen Bereiche sind: Moderne
Anwendungen in der Licht und Elektronenmikroskopie, mikroskopische Untersuchung von lebenden Zellen, organotypische Zellkulturen, epigenetische Analysen, RNAi-Techniken, Genexpressionanalyse, Zellzyklusregulation, Protein-Protein und Protein-DNA Wechselwirkungen, DNA Microarray Techniken und Proteom Analysen.