301052 UE UE in Fluoreszenz und Konfokalmikroskopie inkl.Übungen zur Bild-bearbeitung/analyse (2026S)
Prüfungsimmanente Lehrveranstaltung
Labels
Zusammenfassung
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Do 12.02.2026 14:00 bis Do 26.02.2026 18:00
- Abmeldung bis Do 26.02.2026 18:00
An/Abmeldeinformationen sind bei der jeweiligen Gruppe verfügbar.
Gruppen
Gruppe 1
In case non-German speaking students participate we, of course, will teach in English!
max. 12 Teilnehmer*innen
Sprache: Englisch
Lernplattform: Moodle
Lehrende
Termine
TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!
PRELIMINARY MEETING (DIGITAL VIA ZOOM-see below): Tue, March 10th, 2026, 10h30-11h00amATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Confocal Course SS 2026 Preliminary Meeting
Time: Mar 10, 2026 10:30 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/62749925666?pwd=NhPme3o5zxkLkr96wrTFwREdmaNa0n.1
Meeting ID: 627 4992 5666
Passcode: 760676Course details will be announced in Moodle: https://moodle.univie.ac.at/course/view.php?id=458673!! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301052-1 OR -2) IS SUFFICIENT!!
!! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
- The seats will be preferentially assigned to students of the Master-Program (865, 830, 834 and 877).
- Seat allocation will be performed according to the amount of the points bid. Points bid for the last semester will remain considered!
- The course manager may enact a quota-rule for 2 seats (1 seat/course) for students from studies with a natural science background (other than the one listed above) - allocation of these seats according to the amount of points bid
- @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)Seat limitation: 12 (per course; in total for both courses 24 seats)Details for course program, schedule and workflow in the preliminary meeting.
Final seat and group allocation will be done shortly (2-3 days) after the preliminary meeting.
Further details and information will then be distributed via Moodle.
Any changes will be announced in time!COURSE DATES: VALID FOR BOTH COURSES: MAY11-13, 19,21,27,28, 2026 and JUNE 1,3, 2026
THE FOLLOWING DATES MANDATORY FOR BOTH COURSES: May 11,12,13 and JUNE 1,3 2026
ADDITIONALLY FOR COURSE 1: MAY 19 OR MAY 21
ADDITIONALLY FOR COURSE 2: MAY 27 OR MAY 28
Attendance at ALL these dates specified for the courses is MANDATORY!
Gruppe 2
In case non-German speaking students participate we, of course, will teach in English!
max. 12 Teilnehmer*innen
Sprache: Deutsch, Englisch
Lernplattform: Moodle
Lehrende
Termine
TEACHING LANGUAGE OF THE COURSE IS ENGLISH!!!!
PRELIMINARY MEETING (DIGITAL VIA ZOOM-see below): Tue, March 10th, 2026, 10h30-11h00amATTENDANCE AT THE PRELIMINARY MEETING IS MANDATORY!!!!
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Confocal Course SS 2026 Preliminary Meeting
Time: Mar 10, 2026 10:30 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/62749925666?pwd=NhPme3o5zxkLkr96wrTFwREdmaNa0n.1
Meeting ID: 627 4992 5666
Passcode: 760676Course details will be announced in Moodle: https://moodle.univie.ac.at/course/view.php?id=458673!! REGISTRATION VIA U:SPACE FOR ONE OF THE OFFERED COURSES (301052-1 OR -2) IS SUFFICIENT!!
!! REGISTRATION VIA U:SPACE DOES NOT AUTOMATICALLY LEAD TO COURSE PARTICIPATION!IN CASE OF OVER-BOOKING THE ACTUAL SPL-RULES WILL BECOME APPLIED:
- The seats will be preferentially assigned to students of the Master-Program (865, 830, 834 and 877).
- Seat allocation will be performed according to the amount of the points bid. Points bid for the last semester will remain considered!
- The course manager may enact a quota-rule for 2 seats (1 seat/course) for students from studies with a natural science background (other than the one listed above) - allocation of these seats according to the amount of points bid
- @ points: if a vacant seat cannot become allocated based on points (e.g. same # of points bid), then the date of registration will decide (the earlier the better)Seat limitation: 12 (per course; in total for both courses 24 seats)Details for course program, schedule and workflow in the preliminary meeting.
Final seat and group allocation will be done shortly (2-3 days) after the preliminary meeting.
Further details and information will then be distributed via Moodle.
Any changes will be announced in time!COURSE DATES: VALID FOR BOTH COURSES: MAY11-13, 19,21,27,28, 2026 and JUNE 1,3, 2026
THE FOLLOWING DATES MANDATORY FOR BOTH COURSES: May 11,12,13 and JUNE 1,3 2026
ADDITIONALLY FOR COURSE 1: MAY 19 OR MAY 21
ADDITIONALLY FOR COURSE 2: MAY 27 OR MAY 28
Attendance at ALL these dates specified for the courses is MANDATORY!
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Theoretische Grundlagen der Fluoreszenz- und Konfokalmikroskopie. Technische Erläuterung der verwendeten Geräte Laborübungen Probenvorbereitungen Qualitätskriterien Aberrationskorrekturen Mikroskopieren von ausgewählten Proben mittels Fluoreszenz- und Konfokalmikroskopie (Mehrkanalaufnahmen, serielle optische Schnitte durch Proben, Bildprojektionen, Aufnehmen von Zeitserien) Bildanalyse und Dekonvolution von eigens angefertigten Bildern
Art der Leistungskontrolle und erlaubte Hilfsmittel
continuous assessment
Mindestanforderungen und Beurteilungsmaßstab
The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.
Prüfungsstoff
microscopy sample preparation
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
Literatur
J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006Yuste, Imaging; A Laboratory manual; CSHL-Press, 2010D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Zuordnung im Vorlesungsverzeichnis
MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD (SPL 56)
Letzte Änderung: Fr 16.01.2026 13:27