301272 VO+UE Live imaging course (2026S)
Prüfungsimmanente Lehrveranstaltung
Labels
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Do 12.02.2026 14:00 bis Do 26.02.2026 18:00
- Abmeldung bis Do 26.02.2026 18:00
Details
max. 12 Teilnehmer*innen
Sprache: Englisch
Lehrende
Termine
COURSE LANGUAGE IS ENGLISH!!
VORBESPRECHUNG/PRELIMINARY MEETING: via Zoom - March 10th, 2026; 11h00-11h30amJosef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Live Imaging Course SS2026 Preliminary Meeting
Time: Mar 10, 2026 11:00 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/68758212450?pwd=ybmOtYl1uM7B3pomM4tLf0bgnzeaCn.1
Meeting ID: 687 5821 2450
Passcode: 491958Attendance at the preliminary meeting is MANDATORY, since we expect more people to become registered for the course than seats are available!The course will be held : JULY 7+8, JULY 14 - full day 9.00-17.30.
Location: Campus Vienna Biocenter; Building VBC5, Level E1.
The schedule will be available at Moodle: https://moodle.univie.ac.at/course/view.php?id=458671IMPORTANT INFORMATION
The course may be heavily over-booked for the 12 available seats.
Based on this situation, I will apply the official SPL rules:
- For the allocation of course seats, preference will be given to students of the master studies UA 865, 830, 834, 877.
- Students of the master studies UA 865, 830, 834, 877 will be ranked according to points bet.
- Points bet for the last semester will remain considered!
- The course manager may assign one of the 12 seats to a student of studies with a natural sciences background. If more than one student of these studies registered, a points bet ranking will be enacted as well.More details in the preliminary meeting on March 10th, 2026.
A list of the top12 ranked participants (according to the rules described above) will be announced shortly before the preliminary meeting to make sure these people will participate at the meeting - still, attendance is mandatory not to lose a potential seat in the course!All others will be put on the waiting list. If later seats become vacant, the following rules will be applied
If seats become vacant, the waiting list will be grouped in persons of 5.
The first 5 people (= group1) on the waiting list will be informed - the seats will be assigned then on a "first come first served" basis.
If nobody of the first group replies or reply negatively, the next 5 people on the list (group 2) are considered, and so on and so on.
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Art der Leistungskontrolle und erlaubte Hilfsmittel
Continuous Assessment (60% of the grade)
- writing a protocol and a small exercise (group-wise) after the course is finished (40% of the grade)
- writing a protocol and a small exercise (group-wise) after the course is finished (40% of the grade)
Mindestanforderungen und Beurteilungsmaßstab
Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in extended practical hands-on-sessions using different live microscopy setups. One part of the course is dedicated to data handling from live imaging experiments.
Continuous assessment: 60% of the grade
Protocol and exercise quality: 40% of the grade
Continuous assessment: 60% of the grade
Protocol and exercise quality: 40% of the grade
Prüfungsstoff
a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will be taught how to handle live imaging data in terms of processing and analysis
f. Students will learn and work in cooperative teams
g. a quiz will help evaluate the learning success, as questions are to be answered before the course starts and also at the end of the course
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will be taught how to handle live imaging data in terms of processing and analysis
f. Students will learn and work in cooperative teams
g. a quiz will help evaluate the learning success, as questions are to be answered before the course starts and also at the end of the course
Literatur
Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
www.fpbase.org
https://www.ibiology.org/talks/live-cell-imaging/
http://www.microscopyu.com/
https://www.olympus-lifescience.com/de/microscope-resource/
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/
https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
www.fpbase.org
https://www.ibiology.org/talks/live-cell-imaging/
http://www.microscopyu.com/
https://www.olympus-lifescience.com/de/microscope-resource/
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/
https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html
Zuordnung im Vorlesungsverzeichnis
MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD (SPL 56)
Letzte Änderung: Mo 16.02.2026 17:27
The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using advanced light microscopic technologies, like confocal, spinning disc, fluorescence widefield and even superresolution imaging microscopy.
In total now 7 lectures on individual topics will be held:
- Live Imaging Considerations
- Sample preparation
- Single molecule superresolution microscopy
- theoretical comparison of course microscope modalities
- Dyes and Fluorophores
- Specialized Techniques (FRAP, AIRY SCAN, LATTICE SIM)
- Handling Live Imaging DataThe 12 attendees will become split into three groups of 4 people. Each group will work on one of the three offered microscopy modalities (widefield, confocal, spinning disc) throughout the course.
Extensive hands-on planning of overnight and individual shorter time lapse experiments using various available microscope setups will be considered. Special applications at each modality, such as photomanipulation, superresolution options and/or specialized processing are part of the training.
Two groups of 6 people will have the chance to get a home-built superresolution microscope setup demoed.
The final afternoon is dedicated to give expert advice on how to handle live imaging data - this will include selected examples as well as insight into advanced processing pipelines, including aspects of Artificial Intelligence.