Universität Wien

270036 PR Mass Spectrometry II (digital) (2025W)

3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Continuous assessment of course work
MIXED
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 10 participants
Language: German

Lecturers

Classes

Preliminary meeting on October 16, 2025, 2:00 p.m. - 3:00 p.m. via Zoom (link will be sent by email).
Note: Some of the assignments must also be completed at home. Please take this into account when planning your workload.

Course days:
03.11.2025, from 13:00 | Kleiner Hörsaal 4 (Halbstock, Währinger Straße 42)
04.11.2025, 12:30 - 15:30 | Kleiner Hörsaal 4 (Halbstock, Währinger Straße 42)
10.11.2025, from 12:30 | Seminarraum Köllensperger (Raum 2H41; Halbstock, Währinger Straße 42)
17.11.2025, from 12:30 | Seminarraum Köllensperger (Raum 2H41; Halbstock, Währinger Straße 42)

Please bring a laptop or tablet to all sessions, at least one device per group of two!

Information

Aims, contents and method of the course

IMPORTANT:
- This lab course will be held exclusively in a seminar room, we will not go into any lab.
- Online teaching is only planned in the event of a pandemic or similar situation.
- Please bring a laptop or tablet to all sessions. If you do not have your own device, please form groups of two so that at least one laptop is available per group.

Objectives:
Students will practice analyzing and interpreting extensive mass spectrometry data using a proteomics data set as an example.
By the end of the lab, they will be able to:
- describe and critically evaluate a typical bottom-up proteomics workflow (HPLC-MS) in technical terms,
- evaluate a given data set in a reflective manner using appropriate software,
- place results in a (biological) context, substantiate them with literature, and present them.

Content:
- Fundamentals of HPLC-MS
- Bottom-up proteomics workflow, including quality assurance
- Proteomics software
- Statistics
- Literature research (classification of results)
- Scientific writing (protocol)

Method:
The course consists of theoretical and practical units, supplemented by independent work by the students.
In the theoretical units, the necessary knowledge is conveyed through interactive lectures and live demonstrations.
In addition, students work independently and in small teams, e.g., on literature research, data evaluation, or specific tasks.

Assessment and permitted materials

Assessment criteria:
- Participation and practical work (continuous assessment)
- Group presentation on experimental design
- Written report
- Short debriefing

Permitted aids:
Laptop, tablet, smartphone

Minimum requirements and assessment criteria

Minimum requirements:
- Course with continuous assessment: compulsory attendance throughout the entire lab course (exceptions only with justified and timely excuse)
- Complete and timely submission of all assignments, including a written report
- Completion of all partial assignments by the end of the course

Assessment criteria:
- Active participation
- Quality of the presentation
- Quality of the report
- Quality of the debriefing

Examination topics

Debriefing: Review of understanding and plausibility check of results

Reading list

Will be discussed during the course, if needed.

Association in the course directory

CH-SAS-05, CH-SAS-06, CH-SAS-07, CH-SAS-08, LMC C1, LMC D1, BC-CHE II-1

Last modified: Mo 03.11.2025 17:07