Universität Wien

270175 UE Lab Course: Basic Techniques in Biochemistry (2018S)

for Chemistry Students

5.00 ECTS (5.00 SWS), SPL 27 - Chemie
Continuous assessment of course work

Summary

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 16 participants
Language: English
LMS: Moodle

Lecturers

Classes

Vorbesprechung: 9.3.2018, 14.15 Uhr
ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Termin: 14. - 23.3.2018
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/
Anmeldung über Univis Cluster Biologie

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Assessment and permitted materials

Evaluation criteria: active participation, discussions of the experiments, experimental success, written final exam.
Course script available via moodle (German only)

Minimum requirements and assessment criteria

Comprehension of basic methods in Biochemistry, planing and execution of biochemical experiments.

Group 2

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes (iCal) - next class is marked with N

Vorbesprechung: 9.3.2018, 14.15 Uhr
ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Termin: 11.4. - 20.4.2018
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/
Anmeldung über Univis Cluster Biologie

Friday 09.03. 14:15 - 15:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
Wednesday 11.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Thursday 12.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Friday 13.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Monday 16.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Tuesday 17.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Wednesday 18.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Thursday 19.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Friday 20.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

Minimum requirements and assessment criteria

Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

Group 3

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes (iCal) - next class is marked with N

Vorbesprechung: 9.3.2018, 14.15 Uhr
ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Termin: 23.4. - 4.5.2018
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/
Anmeldung über Univis Cluster Biologie

Friday 09.03. 14:15 - 15:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
Monday 23.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Tuesday 24.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Wednesday 25.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Thursday 26.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Friday 27.04. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Wednesday 02.05. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Thursday 03.05. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien
Friday 04.05. 09:00 - 17:00 BZB-Übungsraum 1B, 6.Ebene 6.521, Dr.-Bohrgasse 9, 1030 Wien

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

Minimum requirements and assessment criteria

Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

Group 4

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

Vorbesprechung: 9.3.2018, 14.15 Uhr
ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Termin: 25.6. - 5.7.2018
Siehe auch Studentenseite der Institute Dr. Bohr-Gasse: http://molekularebiologie.univie.ac.at/
Anmeldung über Univis Cluster Biologie

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Assessment and permitted materials

Evaluation criteria: active participation, discussions of the experiments, experimental success, written final exam.

Course script available via moodle (German only)

Minimum requirements and assessment criteria

Comprehension of basic methods in Biochemistry, planing and execution of biochemical experiments.

Information

Examination topics

Content and Background of the experiments carried out during the lab course

Reading list


Association in the course directory

BA CH 17b

Last modified: Mo 07.09.2020 15:41