Universität Wien FIND

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270175 UE Lab Course: Basic Techniques in Biochemistry (2019S)

for Chemistry Students

5.00 ECTS (5.00 SWS), SPL 27 - Chemie
Continuous assessment of course work

Summary

1 Hamilton , Moodle
2 Hartig , Moodle
3 Hamilton , Moodle
4 Haider , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

Termin: 20.-29.05.2019 (K5)
Vorbesprechung verpflichtend für die Übungsteilnahme: 1.3.19, 15.00 Uhr
BZB/SR1, Dr. Bohr-Gasse 9, 1030 Wien.
Weitere Infos: http://molekularebiologie.univie.ac.at/

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Assessment and permitted materials

Evaluation criteria: active participation, discussions of the experiments, experimental success, written final exam.
Course script available via moodle (German only)

Minimum requirements and assessment criteria

Comprehension of basic methods in Biochemistry, planing and execution of biochemical experiments.

Group 2

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

Vorbesprechung verpflichtend für die Übungsteilnahme: 1.3.19, 15.00 Uhr
BZB/SR1, Dr. Bohr-Gasse 9, 1030 Wien.
Termin: 03.-14.06.2019 (K6)
ganztägig

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

Minimum requirements and assessment criteria

Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

Group 3

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

Vorbesprechung verpflichtend für die Übungsteilnahme: 1.3.19, 15.00 Uhr
BZB/SR1, Dr. Bohr-Gasse 9, 1030 Wien.
Termin: 17.- 28.06.2019 (K7), ganztägig

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

Minimum requirements and assessment criteria

Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

Group 4

max. 16 participants
Language: German
LMS: Moodle

Lecturers

Classes

Termin: 03.-12.07.2019(K8), ganztägig
Vorbesprechung verpflichtend für die Übungsteilnahme: 1.3.19, 15.00 Uhr
BZB/SR1, Dr. Bohr-Gasse 9, 1030 Wien.

Aims, contents and method of the course

Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Assessment and permitted materials

Evaluation criteria: active participation, discussions of the experiments, experimental success, written final exam.

Course script available via moodle (German only)

Minimum requirements and assessment criteria

Comprehension of basic methods in Biochemistry, planing and execution of biochemical experiments.

Information

Examination topics

Content and Background of the experiments carried out during the lab course

Reading list


Association in the course directory

BA CH 17b

Last modified: Mo 04.02.2019 13:48