Universität Wien

270190 UE Laboratory Course: Molecular Biology Techniques (2021W)

6.00 ECTS (6.00 SWS), SPL 27 - Chemie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 20 participants
Language: German

Lecturers

Classes

Vorbesprechung:
Termin: 7.-18.2.22
Siehe auch Studentenseite der Departments:
http://molekularebiologie.univie.ac.at/

Information

Aims, contents and method of the course

This course teaches the first steps in molecular biology research. Students learn to in vitro recombine DNA molecules (cloning), to isolate DNA from bacteria and yeast, to use PCR, and to perform bacterial transduction.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

UPD: A. Minimal requirements: compulsory attendance, active participation, written protocol and final exam. For the positive grade, all partial achievements must be positively assessed. B. Contribution of partial achievements to the final grade: active participation: 33%; labbook and report (protocol): 33%; final written examination: 34%. The final exam must be passed positively; an opportunity for improvement in the case of negative results is possible. C. Assessment criteria. Active participation refers to reading and completion of the materials and activities on course Moodle pages, active participation in experimentation, proactive learning of experimental methods and experimental design, data presentation and analysis, completion of tests and assignments during the practical, contribution to in-class discussions, formulation and utterance of questions and comments, own contribution to course teaching materials. Labbook and report: following formal requirements of labbook keeping; consistent note-taking; exact protocols description, including deviations from protocols; comprehensive annotation of images, graphs and tables; concise description of experimental goal(s), expected results, empirical observations and conclusions; possible explanations when empirical observations do not match the expected results; description of troubleshooting strategy. Final exam: correct and complete answers to questions covering examination topics
87-100%: sehr gut
75-86.99%: 2
63-74.99%: 3
50-62.99% 4
0-49.99%: 5

Examination topics

Concentrations and dilutions, buffers, plasmids and their functional elements, plasmid extraction, PCR, restriction cloning, protein expression, SDS-PAGE, DNA (Sanger) sequencing. For methods used in the practical, it is essential to remember and understand the steps and their significance (what happens at each step). For buffers used during the practical, it is essential to understand the function of each component (remembering the exact concentration of each component is a plus, but not required).

Reading list

Association in the course directory

BIO I-1

Last modified: Sa 08.07.2023 00:21