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300094 UE Proteomics in System Biology (2021S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes

The lab course will take place in the week of April 19th. More details will be send by email in the beginning of the SS21.


Information

Aims, contents and method of the course

The terms "proteome" and "metabolome" (entirety of all active proteins and metabolites in a cell or tissue) was coined 10 years ago in analogy to the "genome" (entirety of all genes). The proteome comprises the full suite of all proteins/enzymes in a cell or a tissue. Because of the diversity and dynamic of the proteome sophisticated bioanalytical methods, especially mass spectrometry, are needed. In the frame of the project the student should learn basics of the technology proteomics and a special mass spectrometric technique for the analysis of proteins (shotgun proteomics for identification and quantification). At the same time the course will impart knowledge about mass spectrometrie in modern biology.

Assessment and permitted materials

Attendance control, oral presentation of techniques and results (30%), participation in practical and discussions (30%), written group protocol (40%).

Minimum requirements and assessment criteria

english

Examination topics

Protein extraction,- digestion, -separation (HPLC and SDS-PAGE), Mass Spectrometry for protein ID and quantification, data mining

Reading list


Association in the course directory

BBO 9, BOE 12, MMB W-2, MMEI III, MBO 7, MEC-9, M-WZB, MES5

Last modified: We 03.03.2021 14:29