300105 UE Course III A - Laboratory Work in Molecular Biology (Methods Moleculargenetics) (2007W)
for the specialization Microbiology/Genetics
Continuous assessment of course work
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Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Weitere Informationen auf der Studentenseite der Departments: http://www.students.mfpl.ac.at
Weitere Informationen auf der Studentenseite der Departments: http://www.students.mfpl.ac.at
Details
Language: German
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Currently no class schedule is known.
Information
Aims, contents and method of the course
Assessment and permitted materials
Minimum requirements and assessment criteria
Examination topics
Reading list
Association in the course directory
BMG 7, BPF 3, B316
Last modified: Fr 31.08.2018 08:56
Content:
Preparation of competent E.coli
E. coli and yeast transformation
DNA isolation from E.coli and A. thaliana
PCR
Cloning of amplified genes into yeast and plant expression vectors
Yeast two hybrid system
Plant protoplast transformation
Expression of GFP (green fluorescent protein) and GFP fusion proteins in yeast and plant cells
Reporter gene expression analysis
Detection of GFP with fluorescence microscopy
Maximal number of participants: 20