Universität Wien FIND

300128 UE Marine Microbial Symbiosis Course (2016S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

recommended:
300281 VO Symbiosis - concepts and model systems
300496 VO Meereskunde

LIMITED NUMBER OF PARTICIPANTS: 10 STUDENTS

Details

max. 10 participants
Language: English

Lecturers

Classes

first meeting: 4.3.2016, 10.15 seminar room marine biology
Friday, 10.15-11.45 seminar room marine biology
two weeks research stay, Guadeloupe: 17.7.-31.7. 2016


Information

Aims, contents and method of the course

This course provides an introduction into the practical field of marine microbial symbiosis research. Emphasis is laid on a multidisciplinary integrative approach, ranging from microbial to organismal biology to ecology. Every course has a specific topic that will be discussed in seminars. Students will learn how to cooperate in small groups, develop a research project, get an overview on the relevant literature, develop hypotheses, research questions and a research plan, organize the field trip, get familiar with underwater field and lab work, carry out practical work under the given circumstances, and present their results in form of oral presentations and a written report. A research station, appropriate for the selected topic will be chosen for a two-weeks field trip.
Topic of course 2016:
Diverse microbial symbioses live in shallow-water, tropical marine environments, from 1) photoautotrophic dinoflagellates and bacteria associated with corals and upside down jellyfish, 2) thiotrophic bacterial symbionts with archea, protists, stilbonematine nematodes, gutless oligochaetes, and lucinid clams 3) multi-species consortia in sponges, 4) heterotroph, anaerobic methanogens and sulfate reducing bacteria in protists, 5) nitrogen fixing bacteria in shipworms. The ecosystems found in Guadeloupe span coral reefs, seagrass meadows, mangroves, intertidal and subtidal sediments, and shallow-water hydrothermal vents.
Goals:
According to ecosystem, we will search for symbioses in situ, and characterize their habitat (location, depth, temperature, salinity, pH, oxygen, sulfide). In selected symbioses, abundances will be studied and in situ and in vivo stress experiments will be designed and carried out.
Methods:
NO scuba diving, snorkeling required (ABC equipment required, diving suite recommended)
Underwater sampling of animals, seagrass, mangrove peat, wood, water and porewater, sediment; underwater photography and video; measurements of temperature, salinity, pH, oxygen, sulfide, nitrate; dissection-, light- and epifluorescence microsopy and photography and video, extraction and filtration techniques for microbes and animals, stainings: DAPI, live/dead bacterial viability, nile red and bodipy staining, biofilm cell stain, flow-through experimental chamber maintenance

Assessment and permitted materials

Evaluation will be according to the quality of the presentation each student has to prepare and present including literature search, participation in practical team work during the research stay and discussions, presentation of results as oral presentations and a written report.

Minimum requirements and assessment criteria

Aim is to get familiar with microbial symbioses and their habitats and to understand the conditions under which they thrive.

Examination topics

Selected topics and methods in marine microbial symbiosis
evaluation: 60% written report, 40% oral presentations, practical work and discussions

Reading list


Association in the course directory

MEC-9, MEV W-1, MZO W-2, MMB W-2, MGE III-2, MMEI III

Last modified: Fr 31.08.2018 08:56