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300156 UE Advanced techniques of electronmicroscopy and ultrastructural research (2018S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 8 participants
Language: German

Lecturers

Classes

Preliminary meeting: Mo., March 5, 2018, 9:15 a.m. in the zoology conference room.
Beginning of the course on Mo., April 9, 2018, starting with an introductory lecture at 8:15 a.m. in the zoology conference room, UZA1.The laboratory work begins at 9:15 a.m. in the Core Facility of Cell Imaging and Ultrastructure Research (Electron- Microscopy- Lab). The whole course lasts 3 weeks en bloc from 09.04. - 27.04.2018. Especially during the first 2 weeks there is a lecture to the course at 8:15 a.m. , the practical course starts at 9:15 a.m.( until 4 p.m.)


Information

Aims, contents and method of the course

The main topic of this electron microscopy course is the detection of heavy metals and other chemical elements in plant- and animal- cells and tissues by means of energy dispersive X-ray microanalysis (EDX). An introduction is also given in electron energy loss spectroscopy (EELS), the use of unstained ultrathin, and of semithin sections in the TEM and SEM. The investigations are made on the filtering TEM Zeiss EM 902 and TEM Zeiss Libra 120, as well as on the digital SEM Philips XL-30 and JEOL JSM IT300. Preparation methods freeze-drying, carbon-coating, ultrathin sectioning by ultramicrotome and contrasting of sections on grids for TEM.

Assessment and permitted materials

Workshop 25%, poster 25%, protocol 25%, oral examination 25%

Minimum requirements and assessment criteria

Presentation of a project on heavy metal contamination and stress of animals and plants. An additional aim is the knowledge of special SEM and TEM-methods and of techniques for sample preparation.

Examination topics

The results of the project- experiments will be discussed and the advanced techniques of electron microscopy will be dealt with , such as EELS, ESI, Contrast Tuning, ESEM, EDX, BSE, Dot-Mapping, semithin and ultrathin sectioning on the Ultramicrotome, advanced SEM and TEM.

Reading list

Will be offered during the course

Association in the course directory

B-WZB, WZB, UF MA BU 04, MZO 2, MEC-9 , MBO 7

Last modified: Mo 07.09.2020 15:43