300218 UE Scanning electron microscopy - techniques and preparations for biologists (2020S)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 06.02.2020 08:00 to Th 20.02.2020 18:00
- Deregistration possible until Th 30.04.2020 18:00
Details
max. 6 participants
Language: German, English
Lecturers
Classes (iCal) - next class is marked with N
Mon. 02.03.2020
8:15 - 09:30 obligatory Safety Instructions
09:30 - 15:00 Lecture part 1
Tue. 03.03.2020
8:15 - 12:00 Lecture part 2
practical work int the lab of electron microscopy (CF Cell Imaging und UltrastrukturforschungThur.12.03.2020 8:15 - 12:00
Final meeting
Short presentations
- Monday 02.03. 08:15 - 15:00 Konferenzraum
- Tuesday 03.03. 08:15 - 12:00 Konferenzraum
- Thursday 12.03. 08:15 - 12:00 Konferenzraum
Information
Aims, contents and method of the course
Assessment and permitted materials
Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.
Minimum requirements and assessment criteria
1) Cooperation (40%)
2) Short presentation (10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (40%)
3) Protocol (10%)
4) oral examination (10%)Every part has to be positive
2) Short presentation (10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (40%)
3) Protocol (10%)
4) oral examination (10%)Every part has to be positive
Examination topics
Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.
Reading list
Will be presented during the course
Association in the course directory
MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7, MZO 2
Last modified: Sa 22.10.2022 00:29
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)