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300218 UE Scanning electron microscopy - techniques and preparations for biologists (2020S)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Details

max. 6 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

Mon. 02.03.2020
8:15 - 09:30 obligatory Safety Instructions
09:30 - 15:00 Lecture part 1
Tue. 03.03.2020
8:15 - 12:00 Lecture part 2

ab Di 03.03.2020 13:00
practical work int the lab of electron microscopy (CF Cell Imaging und Ultrastrukturforschung

Thur.12.03.2020 8:15 - 12:00
Final meeting
Short presentations

Monday 02.03. 08:15 - 15:00 Konferenzraum der Zoologie, UZA 1,Biozentrum Althanstraße 14, 1.015 EG
Tuesday 03.03. 08:15 - 12:00 Konferenzraum der Zoologie, UZA 1,Biozentrum Althanstraße 14, 1.015 EG
Thursday 12.03. 08:15 - 12:00 Konferenzraum der Zoologie, UZA 1,Biozentrum Althanstraße 14, 1.015 EG

Information

Aims, contents and method of the course

Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)

Assessment and permitted materials

Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.

Minimum requirements and assessment criteria

1) Cooperation (40%)
2) Short presentation (10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (40%)
3) Protocol (10%)
4) oral examination (10%)

Every part has to be positive

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Will be presented during the course

Association in the course directory

MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7, MZO 2

Last modified: Mo 07.09.2020 15:22