Universität Wien FIND

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300286 UE Fluorescence in situ hybridization (FISH) (2020W)

Identification of uncultured microorganisms

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Details

max. 12 participants
Language: German

Lecturers

Classes (iCal) - next class is marked with N

Blockveranstaltung: 15.-26.2.2021 im Übungsraum 7/Anatomie

Tuesday 16.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Wednesday 17.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Thursday 18.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Friday 19.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Monday 22.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Tuesday 23.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Wednesday 24.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Thursday 25.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG
Friday 26.02. 09:00 - 17:00 Übungsraum 6, UZA I, Biozentrum Althanstraße 14, 2.208 1.OG
Übungsraum 7 UZA 1, Biozentrum Althanstraße 14, Anatomie 2.212 1.OG

Information

Aims, contents and method of the course

In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.

Assessment and permitted materials

Continuous assessment of course work. Participation during the course, written protocol, seminar presentation, written exam.

Minimum requirements and assessment criteria

Active participation in lab experiments, discussions, and seminar presentation (40%);
Written exam and protocol (60%)

Examination topics

Content of lectures during the course and content of course hand book

Reading list


Association in the course directory

BOE 12, BMB 10, BMG 10

Last modified: Fr 11.09.2020 12:49