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300286 UE Fluorescence in situ hybridization (FISH) (2021W)

Identification of uncultured microorganisms

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: German

Lecturers

Classes (iCal) - next class is marked with N

Blockveranstaltung: 31.1.2022 - 11.02.2022

Tuesday 01.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Wednesday 02.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Thursday 03.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Friday 04.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Monday 07.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Tuesday 08.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Wednesday 09.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Thursday 10.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Friday 11.02. 08:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1

Information

Aims, contents and method of the course

In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.

Assessment and permitted materials

Continuous assessment of course work. Participation during the course, written protocol, seminar presentation, written exam.

Minimum requirements and assessment criteria

Active participation in lab experiments, discussions, and seminar presentation (40%);
Written exam and protocol (60%)

Examination topics

Content of lectures during the course and content of course hand book

Reading list


Association in the course directory

BOE 12, BMB 10, BMG 10

Last modified: Th 02.09.2021 10:09