Universität Wien FIND

300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2016W)

techniques and preparations for biologists

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


Meeting is compulsory, but students can also send representative in their place.

For further questions, please send email to jacqueline.montanaro@univie.ac.at

Details

max. 56 participants
Language: English

Lecturers

Classes

Montag, 31.10.2016, 12.00 in UZ1 Zoology Conference Room.
Meeting is compulsory, but students can also send representative in their place.

Mo. 7.11.2016 8:15 - 12:00 Seminar Room1 UZ1 Lectures, 13:00 – 16:00 Practical work, EM-LAB Core Facility Cell Imaging und Ultrastructure Research

Di. 8.11.2016 8:15 -16:00 Practical work, EM-LAB Core Facility Cell Imaging und Ultrastructure Research

Mi. 9.11.2016 8:15 - 16:00 Practical work, EM-LAB Core Facility Cell Imaging und Ultrastructure Research

Do. 10.11.2016 8:15 - 16:00 Practical work, EM-LAB Core Facility Cell Imaging und Ultrastructure Research

Fr. 11.11.2016 8:15 -10:00 Seminar Raum 1 UZ1 Lectures; 10:00 - 6:00 Practical work, EM-LAB Core Facility Cell Imaging und Ultrastructure Research

Mo. 14.11.2016 8:15 -12:00 Final Discussion and Oral Exam, 12:00 Seminar Raum 1 UZ1


Information

Aims, contents and method of the course

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and to perform immunolabelling at light and electron microscopy level. Including how to prepare semi-thin and ultrathin sections using a Cryoultramicrotome (Tokuyasu method). Immunolabelling and contrasting using negative staining, cryosections and acrylic section will be evaluated and compared using the fluorescence and transmission electron microscope. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed.

Assessment and permitted materials

Participation: 40
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive

Minimum requirements and assessment criteria

Examination topics

Knowledge of the theoretical background (lectures). Application of the technical aspects of immunolabeling for LM and TEM (from the practical part). Applications in examples. Analysis, interpretation and evaluation of results.

Reading list

Will be discussed during the practice.

Association in the course directory

MZO W-1, MBO 7, MMB W-2, MGE III-2, MEV 2,

Last modified: Fr 31.08.2018 08:56