Universität Wien

300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2020W)

techniques and preparations for biologists

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 6 participants
Language: English

Lecturers

Classes

Obligatory attendance on Friday 23.10.2020 for the introductory lecture 10:00-11:00 online .
Please note that this is a block internship, the times of compulsory attendance will be announced during the introductory lecture (the general times are from Monday 09.11.2020 08:45 to Friday 20.11.2020 16:00) in the EM-laboratory of CIUS, UZA1, Level 1, Room 1044. Further details will be explained in the introductory lecture. In addition, about 2 weeks after the end of the course, an evening will be organised for the oral examination on technical aspects of the course and student presentations. If you have any questions, please contact the course director, Dr. Jacqueline Montanaro (jacqueline.montanaro@univie.ac.at).


Information

Aims, contents and method of the course

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform immunolabelling at light (LM) and electron microscopy (EM) levels, and how to prepare semi-thin and ultrathin sections using an ultramicrotome. Advanced methods (Tokuyasu method) at the ultrastructural level will also be discussed. Immunolabelling and contrasting using negative staining and acrylic sections will be evaluated and compared using the fluorescence and transmission electron microscopes. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed. Students participating in this course will gain a good understanding of immunological methods for LM and transmission EM. In addition, students can gain practical experience in preparing samples for electron microscopy and also how to interpret results of immunolabelling experiments.

Assessment and permitted materials

Participation: 40
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive

Minimum requirements and assessment criteria

as above

Examination topics

Knowledge of the theoretical background (lectures). Application of the technical aspects of immunolabeling for LM and TEM (from the practical part). Applications in examples. Analysis, interpretation and evaluation of results.

Reading list

Wird während des Praktikums bekannt gegeben

Association in the course directory

MZO W-1, MBO 7, MMB W-2, MGE III-2,

Last modified: Tu 08.09.2020 13:30