Universität Wien FIND
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300421 UE Plant tissue culture (2006S)

Laboratory course in plant tissue culture

0.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Vorbesprechung: 7.März 2006, 16 Uhr im Seminarraum der Abt.Ökophysiologie
Termine nach Vereinbarung in Vorbesprechung

Details

Language: German

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Information

Aims, contents and method of the course

ECTS-Punkte: 6
Isolated plant cells, organs or tissue are cultivated on nutrient medium under aseptic conditions. In vitro systems are suitable models for studying developmental and differentiation processes. Further applications include plant breeding, production of secondary metabolites, ex situ conservation of genetic resources, genetic transformation, coculture with microorganisms and commercial plant propagation (e.g. orchids).
The aim of this practical is to demonstrate different methods and applications of plant cell and tissue culture. Tissue cultures will be set up starting with the preparation of nutrient media, surface sterilization as well as a basic understanding of how to work aseptically. The use of plant growth regulators on growth and morphogenesis will be studied. Experiments on regeneration and propagation systems (somatic embryogenesis, organogenesis, microcutting, cell suspension, protoplasts, meristem and anther culture), transformation with Agrobacterium tumefaciens (wildtype and gfp-labelled) will be performed and evaluated. In addition, cryopreservation, production of artificial seeds as well as assessment of DNA genetic stability (ploidy level) via flowcytometry will be demonstrated.

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Last modified: Fr 31.08.2018 08:56