300517 SE+UE Theory and Application of Confocal Microscopy (2017W)
3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 07.09.2017 08:00 to Th 21.09.2017 18:00
- Deregistration possible until Tu 31.10.2017 18:00
max. 8 participants
Vorbesprechung am 3.10.2017, 17 Uhr im Seminarraum Cell Imaging u.Ultrastrukturforschung (1.394), Ebene 1, Spange 4, UZA I.
Beginn der LV am 13.11..2017; Block von 13.-15.11.2017 und weitere Termine nach Vereinbarung
Aims, contents and method of the course
After rapid development, the Confocal Laser Scanning Microscope (CLSM) became the most important imaging tool in both organismic and molecular biology. In this course, we present the theoretical background of CLSM, its application in structure analysis, in measurement and quantification of physiological parameters like membrane potential, ion concentrations or enzyme activities as well as current special techniques. In a hands-on-training, we will apply the following techniques: localisation and identification of structures with organelle specific fluorescent dyes and GFP, 3D-reconstruction and animation, 4D analysis, ratio imaging, spectral dye separation. Participants are encouraged to bring their own samples. Positive examination of this lab course is a prerequisite for independent work on the CLSM of the Cell Imaging and Ultrastructure Research Unit.
Assessment and permitted materials
Active participation in the lab course, written evaluation of the knowledge aquired in the lectures, protocol
Minimum requirements and assessment criteria
The participants know the theoretical background, the possibilities and the limitations of confocal laser scanning microscopy. They are able to work with a modern CLSM and they are familiar with techniques for sample preparation and the evaluation of results.
Lectures on the theoretical background of CLSM, Hands-on-training with provided samples, detailed investigation of a sample selected by the student.
Sheppard, C. J. R. & Shotton, D. M. 1997 Confocal laser scanning microscopy. Royal Microscopical Society Microscopy Handbooks. New York: Springer.Hibbs, A. R. 2004 Confocal microscopy for biologists. New York: Kluwer Academic/Plenum Publishers.Hepler, P. K. & Gunning, B. E. S. 1998 Confocal fluorescence microscopy of plant cells. Protoplasma 201, 121 - 157.Lacey, A. J. 1989 Light microscopy in biology: A practical approach. Practical Approach. Oxford: IRL Press.
Association in the course directory
Last modified: Mo 07.09.2020 15:43