Universität Wien FIND

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300641 UE Mass Spectrometry based Proteomics (2016W)

Genetics and biochemistry

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Details

max. 12 participants
Language: German, English

Lecturers

Classes

Preparatory meeting: November 21 4 pm s.t., 2016, SR3 (MFPL 6th floor) - the participation is mandatory, please inform us in advance in case you cannot attend.
Course: Jan 30 - Feb 10, 2017
ÜR/BZB, Dr.Bohrgasse 9, 1030 Wien
Further information will be available on moodle.


Information

Aims, contents and method of the course

We offer a practical university course, which provides training in mass spectrometry (MS)-based proteomics. In this laboratory course (limited to 12 students) we will introduce general concepts of MS based proteomics and discuss criteria that are required to prepare protein samples of sufficient quality and purity for MS analysis. You will perform sample preparation techniques (including affinity purification of proteins, reduction and alkylation of cysteines, digestion with trypsin), followed by RP-HPLC (reversed phase high-pressure liquid chromatography) MS measurement - the operation of the mass spectrometer will be demonstrated. You will also be introduced to MS data analysis using freeware MS data analysis tools such as MaxQuant, SeeMS and Perseus. We will furthermore aim to describe qualitative and quantitative differences between samples by applying various analysis techniques and label free quantification methods.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Examination topics

Reading list


Association in the course directory

MMB I-3, MMB I-4, MMB II-3, MMB II-4, MMB W-2, MGE III-1, MMEI III, MMI III-1

Last modified: Mo 07.09.2020 15:44