Universität Wien

301052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2026S)

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann , Moodle
2 Gotzmann , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

In case non-German speaking students participate we, of course, will teach in English!

max. 12 participants
Language: English
LMS: Moodle

Lecturers

Classes

see german

Group 2

In case non-German speaking students participate we, of course, will teach in English!

max. 12 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german text

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy technical description of equipment Wet lab experience sample preparationQuality criteria for dealingwith aberrations correctionpractical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images.

At the beginning of the course there will be a quiz to screen the existing knowledge on the course topic. The same quiz will be conducted after your course attendance, so that all students can evaluate the learning effect from the course.

Assessment and permitted materials

continuous assessment

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006Yuste, Imaging; A Laboratory manual; CSHL-Press, 2010D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD (SPL 56)

Last modified: Fr 16.01.2026 18:47