Universität Wien FIND

Return to Vienna for the summer semester of 2022. We are planning to hold courses mainly on site to enable the personal exchange between you, your teachers and fellow students. We have labelled digital and mixed courses in u:find accordingly.

Due to COVID-19, there might be changes at short notice (e.g. individual classes in a digital format). Obtain information about the current status on u:find and check your e-mails regularly.

Please read the information on https://studieren.univie.ac.at/en/info.

301171 UE Course III A - Laboratory Work in Molecular Biology (2020S)

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Witte , Moodle
2 Schweighofer , Moodle
3 Sonnleitner , Moodle
4 Campbell , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes (iCal) - next class is marked with N

see german version

Monday 09.03. 10:00 - 11:00 BZB/Seminarraum 4, 6.Ebene 6.506, Dr.-Bohr-Gasse 9, 1030 Wien
BZB/Seminarraum 4, 6.Ebene 6.506, Dr.-Bohr-Gasse 9, 1030 Wien
(Kickoff Class)

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

a. Minimum requirement: participation during the internship, writing a protocol, exam,
b. Assessment standard: participation 50%, minutes 30%, exam 20%

Examination topics

Content of the course held

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 2

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version
compulsory kick off meeting: 10.3.2020, 16:00-17:00h
SR 4/BZB,
Dr.Bohrgasse 9/6th floor, 1030 Wien

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Examination topics

Contents and theoretical background of the course units.

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 3

max. 20 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version cancelled!!!
Further information will be provided by e-mail
compulsory kick off meeting: 12.3.2020, 15:00 SR 1/BZB, Dr.Bohrgasse 9/6.Stock, 1030 Wien

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteins

Maximal number of participants: 16

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Minimum requirements for passing the course: Attendance, protocol, final exam.

Assessment criteria: participation 50%, protocol 30%, written exam 20%

Examination topics

Contents and theoretical background of the course units

Reading list

Current Literature and background information will be made available on Moodle

Group 4

max. 20 participants
Language: English
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar.
Students will perform basic molecular biology techniques including PCR, qPCR, cloning, DNA purification, and western blot. Students will also learn the theory behind these techniques, practical considerations for their implementation, and experimental design. In the end, students must display a comprehensive understanding of why each experiment that was performed in the course, how it was done, and why it was done in that way.

Assessment and permitted materials

Compulsory attendance; The evaluation consists of different performances: active participation, written report, theoretical knowledge including final exam; each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Compulsory attendance; Assessment criteria: active participation 30%, final exam 35%, protocol 35%.

Examination topics

The exam will consistent of questions on the specific experiments performed in the course and the purpose of the experiments. In addition, there will be questions about the basic biology addressed by the experiments.

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Association in the course directory

BMG 5, WZB

Last modified: Mo 07.09.2020 15:22