301171 UE Course III A - Laboratory Work in Molecular Biology (2020S)
Continuous assessment of course work
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Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 06.02.2020 08:00 to Th 20.02.2020 18:00
- Deregistration possible until Th 20.02.2020 18:00
Registration information is available for each group.
Groups
Group 1
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
see german version
Monday
09.03.
10:00 - 11:00
BZB/Seminarraum 4, 6.Ebene 6.506, Dr.-Bohr-Gasse 9, 1030 Wien
BZB/Seminarraum 4, 6.Ebene 6.506, Dr.-Bohr-Gasse 9, 1030 Wien (Kickoff Class)
BZB/Seminarraum 4, 6.Ebene 6.506, Dr.-Bohr-Gasse 9, 1030 Wien (Kickoff Class)
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
a. Minimum requirement: participation during the internship, writing a protocol, exam,
b. Assessment standard: participation 50%, minutes 30%, exam 20%
b. Assessment standard: participation 50%, minutes 30%, exam 20%
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 2
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
see german version
compulsory kick off meeting: 10.3.2020, 16:00-17:00h
SR 4/BZB,
Dr.Bohrgasse 9/6th floor, 1030 Wien
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Contents and theoretical background of the course units.
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 3
max. 20 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
see german version cancelled!!!
Further information will be provided by e-mail
compulsory kick off meeting: 12.3.2020, 15:00 SR 1/BZB, Dr.Bohrgasse 9/6.Stock, 1030 Wien
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteinsMaximal number of participants: 16
e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteinsMaximal number of participants: 16
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
Minimum requirements for passing the course: Attendance, protocol, final exam.Assessment criteria: participation 50%, protocol 30%, written exam 20%
Examination topics
Contents and theoretical background of the course units
Reading list
Current Literature and background information will be made available on Moodle
Group 4
max. 20 participants
Language: English
LMS: Moodle
Lecturers
Classes
see german version
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar.
Students will perform basic molecular biology techniques including PCR, qPCR, cloning, DNA purification, and western blot. Students will also learn the theory behind these techniques, practical considerations for their implementation, and experimental design. In the end, students must display a comprehensive understanding of why each experiment that was performed in the course, how it was done, and why it was done in that way.
Students will perform basic molecular biology techniques including PCR, qPCR, cloning, DNA purification, and western blot. Students will also learn the theory behind these techniques, practical considerations for their implementation, and experimental design. In the end, students must display a comprehensive understanding of why each experiment that was performed in the course, how it was done, and why it was done in that way.
Assessment and permitted materials
Compulsory attendance; The evaluation consists of different performances: active participation, written report, theoretical knowledge including final exam; each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
Compulsory attendance; Assessment criteria: active participation 30%, final exam 35%, protocol 35%.
Examination topics
The exam will consistent of questions on the specific experiments performed in the course and the purpose of the experiments. In addition, there will be questions about the basic biology addressed by the experiments.
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Association in the course directory
BMG 5, WZB
Last modified: Mo 07.09.2020 15:22