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301171 UE Course III A - Laboratory Work in Molecular Biology (2021W)

for the specialization Microbiology/Genetics

10.00 ECTS (8.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Summary

1 Witte , Moodle
2 Sonnleitner , Moodle
3 Schweighofer , Moodle
4 Schweighofer , Moodle
5 Witte , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

a. Minimum requirement: participation during the internship, writing a protocol, exam, b. Assessment standard: participation 50%, minutes 30%, exam 20%

Examination topics

Content of the course held

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 2

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Maximal number of participants: 20

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

a. Minimum requirement: participation during the internship, writing a protocol, exam,
b. Assessment standard: participation 50%, minutes 30%, exam 20%

Examination topics

Content of the course held

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 3

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting: Wednesday 6th October 2021 at 5 p.m. (online with Zoom).
Registered students & students on the waiting list will receive an email with the video link on Tuesday 5th October.

dates of laboratory course:
22 Nov - 03 Dec 2021

accompanying Seminar 301172 is mandatory!

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Examination topics

Contents and theoretical background of the course units.

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 4

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting: Wednesday 6th October 2021 at 5 p.m. (online with Zoom).
Registered students & students on the waiting list will receive an email with the video link on Tuesday 5th October.

dates of the laboratory course:
08 Nov - 19 Nov 2021

accompanying Seminar 301172 is mandatory!

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteins Maximal number of participants: 16

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances:

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)

each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)

Examination topics

Contents and theoretical background of the course units

Reading list

Current Literature and background information will be made available on Moodle

Group 5

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

see german version

Aims, contents and method of the course

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

a. Minimum requirement: participation during the internship, writing a protocol, exam, b. Assessment standard: participation 50%, minutes 30%, exam 20%

Examination topics

Content of the course held

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Association in the course directory

BMG 5

Last modified: Fr 22.10.2021 10:49