301171 UE Course III A - Laboratory Work in Molecular Biology (2023W)
for the specialization Microbiology/Genetics
Continuous assessment of course work
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Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 07.09.2023 14:00 to Th 21.09.2023 18:00
- Deregistration possible until Th 21.09.2023 18:00
Registration information is available for each group.
Groups
Group 1
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Angela Witte
- Ermire Jerliu (Student Tutor)
Classes
Mandatory preliminary meeting: 5.10.2023, 10 a.m., Seminarrau 1/2, Level 6, BZB, Dr. Bohrgasse 9, 1030 Vienna.
Date: 6.11. - 24.11.23
Accompanying seminar 301172 obligatory !
Location: ÜS 1,Level 6, BZB, Dr. Bohrgasse 9, 1030 Vienna.
See also student page of the departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
a. Minimum requirement: participation during the internship, writing a protocol, exam, b. Assessment standard: participation 50%, minutes 30%, exam 20%
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 2
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Mandatory preliminary meeting: Monday 2nd October 2023 17:00h Seminarraum 3, Dr.Bohrgasse 9/6.105
Lab course dates: 16.10. - 3.11.23 whole dayPlace: ÜR/BZB, Dr.Bohrgasse 9,1030 WienAccompanying Seminar 301172 mandatory !
Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Please see also department website for students: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 3
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Elisabeth Sonnleitner
- Sabine Bechter (Student Tutor)
- Benedek Tóth (Student Tutor)
Classes
Verpflichtende Vorbesprechung am 4.10.2023 10:00 SR1
Termin: 6. - 24.11.2023 ganztägig
ÜR/BZB, Dr.Bohrgasse 9,1030 Wien.
Begleitendes Seminar 301172 verpflichtend !
Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genesMaximal number of participants: 18
e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genesMaximal number of participants: 18
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
Minimum requirements for passing the course: Attendance, protocol, final exam.Assessment criteria: participation 50%, protocol 30%, written exam 20%
Examination topics
Contents and theoretical background of the course units.
Reading list
Current Literature and background information will be made available on Moodle
Group 4
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Termin: 27.11.-15.12.23 ganztägig
Ort: ÜR/BZB, Dr.Bohrgasse 9, 1030 Wien.
Begleitendes Seminar 301172 verpflichtend !
Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: e.g. Cloning and expression of certain genes in bacteria, yeast or plants including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern/Southern blotting; bacterial or yeast two-hybrid technology, expression analysis of reporter genes, expression of gfp (encoding green flourescence protein) and genes encoding gfp-fusion-proteins Maximal number of participants: 16
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Contents and theoretical background of the course units
Reading list
Current Literature and background information will be made available on Moodle
Group 5
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
Mandatory preliminary meeting: Monday 2nd October 2023 17:00h Seminarraum 3, Dr.Bohrgasse 9/6.105
Lab course dates: 08.01. - 25.01.2024 whole dayPlace: ÜR/BZB, Dr.Bohrgasse 9,1030 WienAccompanying Seminar 301172 mandatory !
Ort: ÜS 1,Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Please see also department website for students: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Association in the course directory
BMG 5
Last modified: Tu 03.10.2023 11:49