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301171 UE Course III A - Laboratory Work in Molecular Biology (2025W)
for the specialization Microbiology/Genetics
Continuous assessment of course work
Labels
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 11.09.2025 14:00 to Th 25.09.2025 18:00
- Deregistration possible until Th 25.09.2025 18:00
Registration information is available for each group.
Groups
Group 1
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Alois Schweighofer
- Pamela Lahner (Student Tutor)
- Linda Maria Ott (Student Tutor)
Classes
Mandatory preliminary meeting:
Wednesday 1st October 2025 17:00hSeminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504Lab course dates: 20.10. - 7.11.2024 whole dayPlace: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
Accompanying Seminar 301172 mandatory !Please see also department website for students: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 2
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Angela Witte
- Katharina Fink (Student Tutor)
- Elisa Husting (Student Tutor)
Classes
Termin: 10.-28.11.2025 ganztägig
Ort: ÜR/MPL, Dr.Bohrgasse 9/6.Stock, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Group 3
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Elisabeth Sonnleitner
- Sabine Bechter (Student Tutor)
- Benedek Tóth (Student Tutor)
Classes
Verpflichtende Vorbesprechung: 29.9.2025 16:00 SR1
Ort: ÜR/BZB, Dr. Bohrgasse 9, 1030 Wien
Termin: 13.- 30.10.2025 ganztägig
Das Seminar findet zur gleichen Zeit wie der Kurs statt.Ort: ÜS 1, Ebene 6, BZB, Dr. Bohrgasse 9, 1030 Wien.
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar:
e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genesMaximal number of participants: 18
e.g. Cloning and expression of certain genes in bacteria including PCR amplification; Transformation of different model organisms; RNA purification; Western/Northern blotting; expression analysis of reporter genesMaximal number of participants: 18
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
Minimum requirements for passing the course: Attendance, protocol, final exam.Assessment criteria: participation 50%, protocol 30%, written exam 20%
Examination topics
Contents and theoretical background of the course units.
Reading list
Current Literature and background information will be made available on Moodle
Group 4
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Katharina Semrad
- Lilith Kleiber (Student Tutor)
- Klaudia Natalia Nawrocki (Student Tutor)
Classes
Course time: 1. - 19. 12. 2025 full day
Location: ÜR/MPL, Dr.Bohrgasse 9/6.Stock, 1030 Wien.
Accompanying seminar 301172 is mandatory!
IMPORTANT: Mandatory pre-meeting for Seminar (LV 301172-4) and Übungen (UE301171-4) takes place 2. October 2025 from 9:00 bis 11:00 am in seminar room 2 (SR2) (6.507) in Dr. Bohrgasse 9, 6. floor. Attendance is required. Registered students that do not show up will be replaced by persons on the waiting list (that are present).
Aims, contents and method of the course
In this course Übungen IIIA (301171-4) the focus lies on methods for RNA research:
RNA extraction from E.coli, in vitro transcription, RNA-protein binding assays and band- shifts on native PAA gels, ribozyme kinetics, RNA-chaperone Assays, RT-qPCR, denaturing PAA gels, protein expression and purification using affinity chromatography and inteine protein splicing, SDS gels, molekular clonieren, prime-editing in E.coli, in silico primer design;
A scriptum will be uploaded latest a week prior to the start of the course.
RNA extraction from E.coli, in vitro transcription, RNA-protein binding assays and band- shifts on native PAA gels, ribozyme kinetics, RNA-chaperone Assays, RT-qPCR, denaturing PAA gels, protein expression and purification using affinity chromatography and inteine protein splicing, SDS gels, molekular clonieren, prime-editing in E.coli, in silico primer design;
A scriptum will be uploaded latest a week prior to the start of the course.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (20%)
+) written exam (40%):
+) written report in form of an scientific article about the experiments from the practicum (30%)
Important: No group protocol!!!!Permitted material for written exam: calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (20%)
+) written exam (40%):
+) written report in form of an scientific article about the experiments from the practicum (30%)
Important: No group protocol!!!!Permitted material for written exam: calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (20%)
+) written exam (40%):
+) written report in form of an scientific article about the experiments from the practicum (30%)
+) verbal examination and discussion (daily) (20%)
+) written exam (40%):
+) written report in form of an scientific article about the experiments from the practicum (30%)
Examination topics
Contents and theoretical background of the course units, chemical calculations, primer design;
Reading list
Current Literature and background information will be made available on Moodle latest a week prior to course start.
Group 5
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Alois Schweighofer
- Olivia Füßl (Student Tutor)
- Luca Pasek (Student Tutor)
Classes
Mandatory preliminary meeting:
Wednesday 1st October 2025 17:00hSeminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504Lab course dates: 01.12. - 19.12.2025 whole dayPlace: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 Vienna
Accompanying Seminar 301172 mandatory !Please see also department website for students: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Examination topics
Content of the course held
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Association in the course directory
BMG 5
Last modified: Mo 29.09.2025 12:27
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter-reporter assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, application and detection of genome editing by CRISPR/Cas9 in tobacco plants.