301171 UE Course III A - Laboratory Work in Molecular Biology (2026S)
Continuous assessment of course work
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Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 12.02.2026 14:00 to Th 26.02.2026 18:00
- Deregistration possible until Th 26.02.2026 18:00
Registration information is available for each group.
Groups
Group 1
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Elisabeth Sonnleitner
- Sabine Bechter (Student Tutor)
- Ruth Pfefferkorn (Student Tutor)
Classes
Verpflichtende Vorbesprechung am 3.3.2026 14:00 SR1
Termin: 13.-30.4.26 ganztägig
Ort: Übungslabor/MPL, Dr. Bohrgasse 9/6.Stock, 1030 Wien
Begleitendes Seminar 301172 verpflichtend !
Siehe auch Studentenseite der Departments: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 2
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Alois Schweighofer
- Olivia Füßl (Student Tutor)
- Pamela Lahner (Student Tutor)
Classes
Mandatory preliminary meeting: Wednesday 4th March 2026 16:30h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504
Lab course dates: 20.04. - 8.05.2026 whole dayPlace: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 ViennaAccompanying Seminar 301172 mandatory !Please see also department website for students: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (Golden Gate Cloning and conventional Cloning: restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter (GUS, RUBY) assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, induction of bioluminescence in plants, application of genome editing by CRISRP/Cas9 in plants.
Preparation of E.coli competent cells, gene cloning (Golden Gate Cloning and conventional Cloning: restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter (GUS, RUBY) assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, induction of bioluminescence in plants, application of genome editing by CRISRP/Cas9 in plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 3
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Katharina Semrad
- Ayumi Kinast (Student Tutor)
- Klaudia Natalia Nawrocki (Student Tutor)
Classes
see german version
mandatory meeting: 9. März 2026, 18:30, Dr. Bohrgasse 9/6, 6. floor, SR 1 (6.501/6.504)
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):Permitted material for written exam: calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):Permitted material for written exam: calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (20%)
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):
+) verbal/written examination and discussion (daily) (30%)
+) written protocol (30%)
+) written exam (20%):
Group 4
max. 18 participants
Language: English
LMS: Moodle
Lecturers
- Angela Witte
- Katharina Fink (Student Tutor)
- Magdalena Nemetz (Student Tutor)
Classes
see seminar 301172
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 5
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Alois Schweighofer
- Olivia Füßl (Student Tutor)
- Pamela Lahner (Student Tutor)
Classes
Mandatory preliminary meeting: Wednesday 4th March 2026 16:30h Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504
Lab course dates: 22.June - 10.July 2026 whole dayPlace: Teaching Lab A Dr.-Bohr-Gasse 9/6.519 A-1030 ViennaAccompanying Seminar 301172 mandatory !Please see also department website for students: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (Golden Gate Cloning and conventional Cloning: restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter (GUS, RUBY) assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, induction of bioluminescence in plants, application of genome editing by CRISRP/Cas9 in plants.
Preparation of E.coli competent cells, gene cloning (Golden Gate Cloning and conventional Cloning: restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter (GUS, RUBY) assays of transgenic plants, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy, induction of bioluminescence in plants, application of genome editing by CRISRP/Cas9 in plants.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)Permitted material for written exam: own written protocol, calculator (internet access and smartphones are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation and results obtained (10%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
+) verbal examination and discussion (daily) (30%)
+) written protocol (10%)
+) written exam (20%):
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (30%)
Group 6
max. 18 participants
Language: German, English
LMS: Moodle
Lecturers
- Christopher Campbell
- Ananya Dodamani (Student Tutor)
- Katharina Nittnaus (Student Tutor)
Classes
Mandatory preempting: 22.April 2026, 15.00
https://univienna.zoom.us/j/67552611524?pwd=ImEaewYUMxfvKRGd0oa85S8oh537VS.1
Place: Teaching laboratory/Max Perutz Labs, Dr. Bohrgasse 9/6.Floor, 1030 Wien
Accompanying Seminar 301172 is required!
See also the student page of the department: http://molekularebiologie.univie.ac.at/
Aims, contents and method of the course
Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar using different model organisms:
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.
Assessment and permitted materials
Compulsory attendance; the evaluation consists of different performances:+) active participation in experiments and discussions (daily) (33%)
+) written exam (33%)
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (33%)Permitted material for written exam: calculator (internet access, smartphones, and written materials are not allowed)each of the subgrades must have a positive evaluation.
+) written exam (33%)
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (33%)Permitted material for written exam: calculator (internet access, smartphones, and written materials are not allowed)each of the subgrades must have a positive evaluation.
Minimum requirements and assessment criteria
+) active participation in experiments and discussions (daily) (33%)
+) written exam (33%)
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (33%)
+) written exam (33%)
+) written report in form of an scientific article (in English) about a selected experiment from the practicum (33%)
Information
Examination topics
Contents and theoretical background of the course units.
Reading list
Course documents in Moodle Information on the literature available on the slides in Moodle.
Association in the course directory
BMG 5
Last modified: Th 12.03.2026 10:27
Preparation of E.coli competent cells, gene cloning (restriction, ligation, transformation) and PCR, yeast transformation and determination of protein-protein interactions using the yeast two hybrid system, genotyping of transgenic plants by PCR, determination of protein expression by SDS-PAGE and Western blotting, promoter reporter assays of transgenic plants, transformation of plant protoplasts, transient transformation of tobacco plants by agroinfiltration, detection of YFP (yellow fluorescent protein) and YFP-fusion proteins in transiently transformed plants by fluorescence microscopy.