Universität Wien
Warning! The directory is not yet complete and will be amended until the beginning of the term.

301172 SE Seminar to Laboratory Course III A (2025W)

for the specialization Microbiology/Genetics

2.00 ECTS (1.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Schweighofer , Moodle
2 Witte , Moodle
3 Sonnleitner , Moodle
4 Semrad , Moodle
5 Schweighofer , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting:

Wednesday 1st October 2025 17:00h
Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

Seminar dates and venue:
15th October 2025 09:00 - 18:00h Seminar Room 2, Dr.Bohr-Gasse 9/6.507
16th October 2025 09:00 - 18:00h Seminar Room 3, Dr.Bohr-Gasse 9/6.105

Aims, contents and method of the course

a) Aims:
+) as presenter to prepare a selected topic (selected molecular biology methods; will be assigned) for a presentation in English (“learning by teaching”).
+) as listener to contribute constructively in the following discussion after the presentation about presentation content and presentation technique.
b) content
Seminar topics (selection): E.coli transformation and cloning vectors, PCR, reporter genes, yeast transformation and yeast two hybrid system, transient and stable transformation of plants, plasmid DNA isolation from bacteria, restriction and modification enzymes, gateway cloning, golden gate cloning, Western Blotting, Southern blotting, CRISPR/Cas9 system, etc
c) methods
Presentations followed by discussion (questions & answers) from the audience; feedback about presentation (technique) will be provided

Assessment and permitted materials

Compulsory attendance.
the evaluation consists of different performances:
+) written exam at end of seminar (contains all presenation topics):
Assessment criteria: 60%
Permitted material: calculator (smartphones are not allowed)
+) presentation (quality and completeness of the seminar presentation, preparation, content, correctness etc)
Assessment criteria: 30%
+) active participation in the scientific discussion of the presented publications
Assessment criteria: 10%

each of the subgrades must have a positive evaluation

Minimum requirements and assessment criteria

compulsatory attendance
giving a presentation (30%)
co-work (10%)
final written exam (60%)

Examination topics

Contents and theoretical background of the course units (content of presentations).

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 2

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting: 2 October 2025, 9 a.m., seminar room 3, level 6, BZB, Dr. Bohrgasse 9, 1030 Vienna

Seminar only together with UE IIIA 301171, registration of topics for the seminar at the preliminary meeting
Dates:
20.10.2025, from 3 p.m, seminar room no.3, Dr. Bohrgasse 9, 6th floor, 1030 Vienna
21.10.2025, from 3 p.m, seminar room no.2, Dr. Bohrgasse 9, 6th floor, 1030 Vienna
22.10.2025, from 3 p.m. seminar room no.2, Dr. Bohrgasse 9, 6th floor, 1030 Vienna
23.10.2025, from 3 p.m, seminar room no. 2, Dr. Bohrgasse 9, 6th floor, 1030 Vienna
24.10.2025, from 3 p.m., seminar room no.2, Dr. Bohrgasse 9, 6th floor, 1030 Vienna

Aims, contents and method of the course

a) Aims:
+) as presenter to prepare a selected topic (selected molecular biology methods; will be assigned) for a presentation in English (“learning by teaching”).
+) as listener to contribute constructively in the following discussion after the presentation about presentation content and presentation technique.
b) content
Seminar topics (selection): E.coli transformation and cloning vectors, PCR, reporter genes, yeast transformation and yeast two hybrid system, transient and stable transformation of plants, plasmid DNA isolation from bacteria, restriction and modification enzymes, gateway cloning, golden gate cloning, Western Blotting, Southern blotting, CRISPR/Cas9 system, etc
c) methods
Presentations followed by discussion (questions & answers) from the audience; feedback about presentation (technique) will be provided

Assessment and permitted materials

Compulsory attendance.
the evaluation consists of different performances:
+) written exam at end of seminar (contains all presenation topics):
Assessment criteria: 60%
Permitted material: calculator (smartphones are not allowed)
+) presentation (quality and completeness of the seminar presentation, preparation, content, correctness etc)
Assessment criteria: 30%
+) active participation in the scientific discussion of the presented publications
Assessment criteria: 10%

each of the subgrades must have a positive evaluation

Minimum requirements and assessment criteria

compulsatory attendance
giving a presentation (30%)
co-work (10%)
final written exam (60%)

Examination topics

Contents and theoretical background of the course units (content of presentations).

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 3

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Verpflichtende Vorbesprechung: 29.9.2025 16:00 SR1
Ort: SR1/MPL, Dr.Bohrgasse 9/6.Stock,1030 Wien

Aims, contents and method of the course

a) Aims:+) as presenter to prepare a selected topic (selected molecular biology methods; will be assigned) for a presentation in English (“learning by teaching”).+) as listener to contribute constructively in the following discussion after the presentation about presentation content and presentation technique.b) contentSeminar topics (selection): E.coli transformation and cloning vectors, PCR, reporter genes, yeast transformation and yeast two hybrid system, transient and stable transformation of plants, plasmid DNA isolation from bacteria, restriction and modification enzymes, gateway cloning, golden gate cloning, Western Blotting, Southern blotting, CRISPR/Cas9 system, etcc) methodsPresentations followed by discussion (questions & answers) from the audience; feedback about presentation (technique) will be provided

Assessment and permitted materials

Minimum requirements for passing the course: Attendance, contributions to discussions at colleagues' presentations, presentation, preparation of documents for the presentation, final exam.

Grading: Presentation 30%, Participation 10%, final exam 60%

each of the subgrades must have a positive evaluation

Minimum requirements and assessment criteria

compulsatory attendancegiving a presentation (30%)co-work (10%)final written exam (60%)

Examination topics

Contents and theoretical background of the course units

Reading list

Green MR and Sambrook J, Molecular cloning: A Laboratoty Manual, Cold Spring Harbor, NY: Cold Spring Harbor Lab. Pr, 4th ed.
Mülhardt C, Der Experimentator: Molekularbiologie Genomics, Springer Spektrum
Müller HJ and Röder T, Der Experimentator: Microarrays, Springer Spektrum
Rehm H and Letzel T, Der Experimentator: Proteinbiochemie/Proteomics, Springer Spectrum

Group 4

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory pre-meeting for Seminar (LV 301172-4) and Übungen (UE301171-4) takes place 2. October 2025 from 9:00 bis 11:00 am in seminar room 2 (SR2) (6.507) in Dr. Bohrgasse 9, 6. floor. Attendance is required. Registered students that do not show up will be replaced by persons on the waiting list (that are present).
Time, Location:
Thursdays 10:00 – 12:00
SR1,2,3 are located at Max Perutz Laboratories, Dr. Bohrgasse 9/6 floor
VBC 5 lecture room at VBC 5 building, 1 floor downstairs;

16. 10. 2025, SR 2 (6.507), 6. Stock, 10:00-12:00
23. 10. 2025, SR1 (6.501/6.504), 6. Stock, 10:00 – 12:00
30.10. 2025, SR3 (6.105), 6. Stock, 10:00 – 12:00
6. 11. 2025, SR1 (6.501/6.504), 6. Stock, 10:00 – 12:00
13.11. 2025, SR1 (6.501/6.504), 6. Stock, 10:00 – 12:00
20. 11. 2025, VBC 5 lecture room A, (1.216), 10:00 – 12:00
27. 11. 2025, VBC5 lecture room A, (1.216), 10:00 – 12:00
8. 1. 2026, SR2, (6.507), 6. Stock, 10:00-12:00

Aims, contents and method of the course

a) Aims:
+) as presenter to prepare a selected topic (selected molecular biology methods; will be assigned) for a presentation in English (“learning by teaching”).
+) as listener to contribute constructively in the following discussion after the presentation about presentation content and presentation technique.
b) content
Seminar topics (selection): E.coli transformation and cloning vectors, PCR, reporter genes, yeast transformation and yeast two hybrid system, transient and stable transformation of plants, plasmid DNA isolation from bacteria, restriction and modification enzymes, gateway cloning, golden gate cloning, Western Blotting, Southern blotting, CRISPR/Cas9 system, etc
c) methods
Presentations followed by discussion (questions & answers) from the audience; feedback about presentation (technique) will be provided

Assessment and permitted materials

Compulsory attendance.
the evaluation consists of different performances:

+) presentation (quality and completeness of the seminar presentation, preparation, content, correctness etc)
Assessment criteria: 60%
+) active participation in the scientific discussion of the presented publications and short exams after presentations
Assessment criteria: 30%
+) Preparation of a 1 page handout (PRIOR to the presentation) maximum 1 page where the topic is explained (clear for everyone)
Assessment criteria: 10%

each of the subgrades must have a positive evaluation

Minimum requirements and assessment criteria

see above

Examination topics

Contents and theoretical background of the course units (content of presentations).

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Group 5

max. 18 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Mandatory preliminary meeting:

Wednesday 1st October 2025 17:00h
Seminar Room 1 Dr.-Bohr-Gasse 9/6.501-6.504

Seminar dates and venue:
26th November 2025 09:00 - 18:00h Seminar Room 2, Dr.Bohr-Gasse 9/6.507
27th November 2025 09:00 - 18:00h Seminar Room 2, Dr.Bohr-Gasse 9/6.507

Aims, contents and method of the course

a) Aims:
+) as presenter to prepare a selected topic (selected molecular biology methods; will be assigned) for a presentation in English (“learning by teaching”).
+) as listener to contribute constructively in the following discussion after the presentation about presentation content and presentation technique.
b) content
Seminar topics (selection): E.coli transformation and cloning vectors, PCR, reporter genes, yeast transformation and yeast two hybrid system, transient and stable transformation of plants, plasmid DNA isolation from bacteria, restriction and modification enzymes, gateway cloning, golden gate cloning, Western Blotting, Southern blotting, CRISPR/Cas9 system, etc
c) methods
Presentations followed by discussion (questions & answers) from the audience; feedback about presentation (technique) will be provided

Assessment and permitted materials

Compulsory attendance.
the evaluation consists of different performances:
+) written exam at end of seminar (contains all presenation topics):
Assessment criteria: 60%
Permitted material: calculator (smartphones are not allowed)
+) presentation (quality and completeness of the seminar presentation, preparation, content, correctness etc)
Assessment criteria: 30%
+) active participation in the scientific discussion of the presented publications
Assessment criteria: 10%

each of the subgrades must have a positive evaluation

Minimum requirements and assessment criteria

compulsatory attendance
giving a presentation (30%)
co-work (10%)
final written exam (60%)

Examination topics

Contents and theoretical background of the course units (content of presentations).

Reading list

Course documents in Moodle Information on the literature available on the slides in Moodle.

Association in the course directory

BMG 4

Last modified: Tu 30.09.2025 12:47