301432 UE RNA Profiling in Microbiology/Immunology (2020W)
Continuous assessment of course work
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Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 10.09.2020 08:00 to Th 24.09.2020 18:00
- Deregistration possible until Sa 31.10.2020 18:00
Details
Information
Aims, contents and method of the course
The course communicates theoretical and practical knowledge necessary to quantify nucleic acid sequences using reverse-transcription quantitative PCR (RT-qPCR), but also digital PCR, the current gold standard for precise counting of (low) DNA copy numbers. Examples from the fields of microbiology and immunology will be addressed. Major experimental topics are the use of reference sequences like spike-in controls for molecular diagnostics, "normalizers" required to determine relative transcript expression and counting of absolute numbers of DNA copies. Practical training includes primer design for monitoring of targets like mRNA variants, micro RNAs and DNAs by a fluorescent dye or various probe formats. The course also aims at developing basic skills and abilities in statistical evaluation and graphical presentation of the data, as well as trains storytelling in written communication.
Assessment and permitted materials
Evaluation consists of different performances:
• Answers in an initial questionnaire (questions are based on information provided by the review article on qPCR listed in the Literature section),
• Active participation and results obtained,
• Theoretical knowledge including oral presentation of a methods article,
• Colloquium on assay design and data evaluation,
• Protocol report (scientific article about one of the data sets or assay designs, 1 page)
• Answers in an initial questionnaire (questions are based on information provided by the review article on qPCR listed in the Literature section),
• Active participation and results obtained,
• Theoretical knowledge including oral presentation of a methods article,
• Colloquium on assay design and data evaluation,
• Protocol report (scientific article about one of the data sets or assay designs, 1 page)
Minimum requirements and assessment criteria
• Basic understanding of the principles of reverse-transcription quantitative PCR and digital PCR including assay design and application to experimental questions in the field of Microbiology and Immunology
• Compulsory attendance
• 100 points can be reached
- Answering the introductory questionnaire 20 points (20%)
- Active participation: 20 points (20%)
- Presentation of a “Methods” article: 20 points (20%)
- Protocol on one data set or assay design (protocol style: draft of a science paper, 1 page):
40 points (40%)All subgrades must have a positive evaluation.
• Compulsory attendance
• 100 points can be reached
- Answering the introductory questionnaire 20 points (20%)
- Active participation: 20 points (20%)
- Presentation of a “Methods” article: 20 points (20%)
- Protocol on one data set or assay design (protocol style: draft of a science paper, 1 page):
40 points (40%)All subgrades must have a positive evaluation.
Examination topics
Review article on quantitative PCR (see literature), content of the presentation and the protocol on one experimental data set or assay design.
Reading list
Article to prepare for the course: Kralik P & Ricchi M (2017) A Basic Guide to Real-Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything. Front Microbiol 8: 108)
Association in the course directory
MGE III-2, MMEI III, WZB
Last modified: We 21.04.2021 11:27