Universität Wien

301503 UE Practical Skills for Microbiome Science II (2025W)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Für diese LV muss vor Kursbeginn (nach Ablauf der Anmeldephase und Aufnahme der Studierenden) eine verpflichtende Sicherheitsunterweisung absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.
There will be a mandatory safty instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
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Moodle
Mo 09.02. 08:00-18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 20 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

see German version

  • Tuesday 10.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 11.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 12.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 13.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Monday 16.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Tuesday 17.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 18.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 19.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 20.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Monday 23.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Tuesday 24.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Wednesday 25.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Thursday 26.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1
  • Friday 27.02. 08:00 - 18:15 Seminarraum 1.4, Biologie Djerassiplatz 1, 1.013, Ebene 1
    Übungsraum 1.6 Molekularbiologie 1, Biologie Djerassiplatz 1, 1.043, Ebene 1

Information

Aims, contents and method of the course

The overarching goal of this course is to learn how we can study microbiomes, including the limitations. The learning objectives for this course are: (1) conceptual understanding of microbial consortia and methods to investigate them and (2) develop technical skills in the lab stemming from basic microbiology to molecular biology and bioinformatic skills. The students will learn such concepts as (1) understanding factors that govern a microbe’s/microbial consortium’s success in the environment, (2) methods to measure their growth/activity, (3) understanding the basic nutrients a cell needs for growth, (4) experimental design concept and (5) documentation of experiments and results. Technical skills include basic microbiology skills, molecular biology skills, single-cell analysis and basic microscopy, along with basic bioinformatic skills.

Methods: The course will be composed mainly of practical experiments, combined with short lectures.
Supervised hands-on training in independent experiments will teach you how to enrich for a targeted physiology, evaluate the function of that enrichment and characterize the community via amplicon sequencing analysis, digital PCR and fluorescence in situ hybridization techniques (FISH). You will further isolate representative strains from the enrichments and characterize the organisms. The course includes computer-based sequence analysis as well as presentations and discussion of the results.
Theoretical knowledge will be disseminated through lectures, data and theoretical discussion sessions in the lab and the course handouts.

Assessment and permitted materials

Evaluation will have four parts: (1) practical participation in the lab, (2) weekly quizzes, (3) final presentation and (4) written report.

Minimum requirements and assessment criteria

Active participation in lab experiments, positive evaluations in weekly quizzes, final presentation, as well as written protocol.

Examination topics

As above described under Methods.

Reading list

Brock Biology of Microorganisms
Further literature will be provided during the course.

Association in the course directory

MMIC 1

Last modified: Mo 03.11.2025 16:07