Universität Wien FIND

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301901 VO+UE Introduction into 3D cryo electron microscopy (2020S)

for diploma, PhD and advanced students

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work



max. 25 participants
Language: English



see german version


Aims, contents and method of the course

Mathematical Background
Fourier Theory for 3D EM
Correlation and Convolution
Information theory for 3D EM

Optics of the Transmission electron microscope
The electron source
The condenser system
The objective and image generation within an electron microscope
Electron detection

Sample preparation

Image processing
Single particle 2D analysis
Single particle 3D generation
Single particle refinement and validation
Tomographic processing

Modern concepts

Negative staining EM
Cryo EM
Tomographic acquisition
Single particle image processing
Tomographic image processing

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, theoretical knowledge including final exam.

Minimum requirements and assessment criteria

Compulsory attendance;
A basic understanding of the concepts presented should be acquired.
Active participation and results obtained,
Theoretical knowledge (final exam);
(The percentage of the subgrades will be announced by the course leader, each of the subgrades must have a positive evaluation.)

Examination topics

Content of the course units

Reading list

Association in the course directory


Last modified: Tu 11.02.2020 11:49