Universität Wien

270052 VO Chemische Biologie (2016W)

3.00 ECTS (2.00 SWS), SPL 27 - Chemie

Details

max. 50 Teilnehmer*innen
Sprache: Englisch

Prüfungstermine

Lehrende

Termine

BEGINN: 4. Oktober, 10.15 Uhr/ Seminarraum 2,
jeweils Dienstag 10.15 12 Uhr, Seminarraum 2 (beim Dekanat für Chemie, Währinger Str. 42, 1. Stock)


Information

Ziele, Inhalte und Methode der Lehrveranstaltung

Website of the lecture: http://www.univie.ac.at/biologischechemie1/chembio/

Teaching Methods
- formal lectures with powerpoint slides
- exercises to re-cap previous lecture
- group discussion and report back on journal articles
- data analysis exercises
- class discussion activities (mind-mapping, peer-teaching etc.)
- "homework"; reading journal articles (about 4-5 over the course)

Learning Outcomes - At the end of the course, students should:
- be familiar with the topics encompassed by the field of chemical biology
- be aware of recent developments in the field
- understand some of the challenges and controversies in the field
- be able to read, understand and critically evaluate a journal article
- be able to discuss a journal article or data set and draw conclusions from it
- be able to apply the knowledge and skills learnt to their master theses and in other situations

Topics:

1. Introduction to chemical biology (mostly AC)
-What is chemical biology? - topics, themes, techniques
-Introduction to chemical biology literature (journals, search engines, strategies for reading journal articles)
-The drug discovery pipeline - process, problems, examples
-Molecules in chemical biology - small molecules, peptides, proteins, antibodies

2. Basic pharmacology and molecular targeted therapies (CB)

- targeted vs non-targeted therapies
- activity, affinity, inhibitors, agonists, antagonists etc.
- drug discovery techniques - natural product screening, combinatorial libraries, chemical genetics...?

3. Protein kinases as anticancer drug targets (CB) -> paper for discussion
- What are protein kinases, active site and mechanism of catalysis
- Why kinases as drug targets? Selectivity and example of marketed drugs
- Known synthesis of used kinase inhibitors

4. GPCRs as drug targets (CB)
- GPCRs: structure and activation
- Examples of GPCRs and chemical biology studies
- Development of proteinase activated receptors inhibitors

5. Therapeutic antibodies and antibody mimetics (CB)
- Antibodies structure and immune response
- Therapeutic antibodies
- Binding proteins

6. Glycans in medicine and vaccine development (CB) -> paper for discussion
- Carbohydrate-based vaccines: challenges and design
- Tumor associated carbohydrate antigens
- Vaccine development and strategies for augmenting T-cell response

7. Antibiotics (CB)
- Antibiotic classification and mechanism
- Strategies for discovering new antibiotics

8. Peptides as drugs (AC)
- advantages and disadvantages of peptide drugs, examples
- strategies for improving peptide stability, permeability and activity

9. Bioactive peptides in nature (AC) -> paper for discussion
- defensins - plant, insect, mammalian
- antimicrobial peptides
- toxins - venom peptides

10. Peptide and protein structure and function - part 1 (AC)
- protein structure - primary, secondary, tertiary, quaternary, intrinsically disordered proteins
- techniques for protein structure determination- CD, NMR, X-ray, FRET, cryo-EM

11. Peptide and protein structure and function - part 2 (AC) -> paper for discussion
- specific examples of protein structure determination using the techniques above
- protein dynamics

12. Protein design (AC)
- de-novo design of proteins and enzymes
- Re-cap and conclusion

Art der Leistungskontrolle und erlaubte Hilfsmittel

Mindestanforderungen und Beurteilungsmaßstab

Assessment

- attendance at > 9 lectures is required. Students are also required to attend the final presentations of their colleagues.
- 10% of the course grade will be based on participation during the course and in the final presentations.
- 90% of the course grade will be awarded for the final presentation. Students will select a journal article from a list provided and will prepare a 10 minute oral presentation with 8-10 slides on the aims and background of the article, a summary of the results obtained and conclusions drawn, and a critical evaluation of the article. The student should then respond to 2 - 3 questions from the lecturers and their colleagues. The presentation will be evaluated as follows: content (40%); presentation style, time (10 ± 2min) and slides (20%); response to questions (20%); and critical evaluation of the journal article (10%).
- grades will be given as: 0 - 49% (5), 50 - 59% (4), 60 - 69% (3), 70 - 79% (2), 80 - 100% (1).

Prüfungsstoff

Literatur


Zuordnung im Vorlesungsverzeichnis

BC-3, BIO II-3, CHE II-3

Letzte Änderung: Mo 07.09.2020 15:41