Universität Wien FIND
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270171 PR Forschungspraktikum Lipidomics (2020W)

3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Prüfungsimmanente Lehrveranstaltung

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

max. 12 Teilnehmer*innen
Sprache: Deutsch

Lehrende

Termine

14th to 18th of December, protocols 4 week later and examination 6 weeks later (mid January)


Information

Ziele, Inhalte und Methode der Lehrveranstaltung

This course will introduce you to the basic concept of lipidomics and will contain lectures but also a lot of practical lipidomics work ranging from sample extraction over the analysis of different lipid classes to their identification and visualization within a given lipidome. More explicitly you will learn about the do and don’ts of lipid sample extraction, familiarize you to the concepts of bottom-up and top-down shotgun lipidomics, analyze your own samples with direct infusion experiments, analyze your data with the latest software and compare your results across different experiments.

Art der Leistungskontrolle und erlaubte Hilfsmittel

We will judge your course work and commitment and questionnaire your work in oral examination.

Mindestanforderungen und Beurteilungsmaßstab

Basic knowledge in data interpretation and statistics, concepts of mass spectrometry (lecture on mass spectrometry)

Prüfungsstoff

practical exertion of experiments and teaching material

Literatur

Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics (doi: 10.1194/jlr.D700041-JLR200 )
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.( doi: 10.1021/ac062455y)
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.( doi: 10.1021/ac102505f)
LipidXplorer: a software for consensual cross-platform lipidomics. (doi: 10.1371/journal.pone.0029851)
Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.( doi: 10.1182/blood-2017-12-822890.)

Zuordnung im Vorlesungsverzeichnis

AN-2

Letzte Änderung: Mi 02.12.2020 09:08