Universität Wien

270171 PR Forschungspraktikum Lipidomics in der Signaltransduktion (2022S)

3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Prüfungsimmanente Lehrveranstaltung

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

max. 12 Teilnehmer*innen
Sprache: Deutsch

Lehrende

Termine

Freitag, 10.06.- Freitag 17.06.2022 im Praktikumssaal 1
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Information

Ziele, Inhalte und Methode der Lehrveranstaltung

Lipidomics is the science that deals with the concentration-dependent description of hydrophobic biomolecules in space and time in a specific system. You will be taught one of the most practical approaches to achieve this goal. This course focuses on LC-MS based lipidomics. However, this course will also deliver basic concepts how to identify small molecules, which you can also apply in other fields of analytical chemistry. In this course the following topics might be presented or worked out together: lipid structure and function, nomenclature of lipids, extraction and sample preparation, LC-MS lipidomics, evaluation strategies for identification and quantification. Critical examination and scientific discussion of research results.

Art der Leistungskontrolle und erlaubte Hilfsmittel

We will judge your course work and commitment and questionnaire your work in oral examination.

Mindestanforderungen und Beurteilungsmaßstab

Basic knowledge in data interpretation and statistics, concepts of mass spectrometry (lecture on mass spectrometry)

Prüfungsstoff

practical exertion of experiments and teaching material

Literatur

Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics (doi: 10.1194/jlr.D700041-JLR200 )
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.( doi: 10.1021/ac062455y)
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.( doi: 10.1021/ac102505f)
Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.( doi: 10.1182/blood-2017-12-822890.)

Zuordnung im Vorlesungsverzeichnis

AN-2

Letzte Änderung: Mi 13.04.2022 13:29