300153 UE Übung zur Entwicklungsbiologie der Pflanzen (2009S)

I. Media preparation, introduction to tissue culture
II. Plant cell cultures in vitro to study morphogenesis and embryogenesis:

- Direct embryogenesis in isolated and cultured tobacco microspores (dicot model);
- Embryogenesis and plant regeneration in isolated wheat microspores (monocot model);
- Embryogenesis in isolated Brassica microspores
- Microspore cultures of Arabidopsis
- Morphogenesis in cultured tobacco leaf discs: impact of growth regulators on the mode of development

III. Basics of light and fluorescence microscopy to study cultured and
developing plant cell:

- Light and fluorescence microscopy to study in vitro cultured tobacco and
Brassica microspores at different developmental stages.

IV. Staining techniques used in plant cell biology to study cell viability,
nuclear status, cell wall and callose)

- FDA staining of isolated microspores, and pollen to study cell viability;
- DAPI staining of microspores and pollen to study the nucleus status;
- Calcofluor White and Aniline Blue staining of pollen and pollen tube to visualize cell wall and callose.