300310 UE Chromosomenanalyse in Blütenpflanzen (2011S)
Chromosomenanalyse in Blütenpflanzen
Prüfungsimmanente Lehrveranstaltung
Labels
Termine der Lehrveranstaltung: Vorbesprechung: 4.03. Kurs: voraussichtlich zweite Hälfte März (Block) im Cytogenetics Labor (Dept. für Bot. Systematik und Evolutionsforschung, Rennweg 14, 1030 Wien)Due to limited number of free places, in case of too many applications priority will be given to the students who took (and graduated) accompanying lecture course ("Plant chromosome evoltuion: from chromosome counting to chromosome painting") in previous years (e.g., SS 2010, VO 300 335), have some theoretical knowledge/practical experience with chromosome analyses, or plan future research (MSc or PhD thesis) in this field.
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Mo 07.02.2011 08:00 bis Di 22.02.2011 20:00
- Abmeldung bis Mi 30.03.2011 20:00
Details
max. 7 Teilnehmer*innen
Sprache: Englisch
Lehrende
Termine (iCal) - nächster Termin ist mit N markiert
- Freitag 04.03. 12:00 - 13:00 Übungsraum 3 (Fakultätszentrum für Biodiversität) Rennweg 1.OG
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Der praktische Kurs führt in die Methoden der Chromosomenpräparation sowie in die klassischen und molekularen Chromosomenfärbetechniken ein. DieTechnik der DNA:DNA in-situ hybridization (FISH), Präparation und Labelling von "DNA probes" (artspezifischer repetitiver DNA, rDNA, telomerische Sequenzen) zur Feststellung von "hybridization sites" mittels Fluoreszenz wird ebenso vermittelt wie die Analyse und Interpretation der erarbeiteten Daten.
Art der Leistungskontrolle und erlaubte Hilfsmittel
Coursework (experiments) 60%
Active participation in experiment planning and results analyses 20%
Written report (in form of manuscript) 20%
Active participation in experiment planning and results analyses 20%
Written report (in form of manuscript) 20%
Mindestanforderungen und Beurteilungsmaßstab
- To introduce modern techniques used nowadays to study the structuure and function of plant genomes and to emphasize the importance of such analyses as complementary approach for phylogenetic, evolutionary, or populational studies.
- To introduce methods for in situ-hybridization probe preparation and labelling, chromosome preparation and quality checks, and finally in situ hybridization
Students can further apply this knowledge to analyse and to interpret obtained results (microscopy).
- To introduce methods for in situ-hybridization probe preparation and labelling, chromosome preparation and quality checks, and finally in situ hybridization
Students can further apply this knowledge to analyse and to interpret obtained results (microscopy).
Prüfungsstoff
- Planning of the experiments; material pre-treatment and fixation; preparation of chromosome spreads using classical methods; documentation.
- Enzymatic chromosome preparations; DNA probe preparation and labelling (PCR labelling, nick translation); preparation of buffers and solutions for FISH.
- Checking of the quality of DNA probe labelling; FISH (fluorescent in situ hybridization) – pretreatments, and hybridization.
- FISH – washing, detection and amplification.
- Analysis of FISH data (microscopy)
- Enzymatic chromosome preparations; DNA probe preparation and labelling (PCR labelling, nick translation); preparation of buffers and solutions for FISH.
- Checking of the quality of DNA probe labelling; FISH (fluorescent in situ hybridization) – pretreatments, and hybridization.
- FISH – washing, detection and amplification.
- Analysis of FISH data (microscopy)
Literatur
Maluszynska J. (ed.) 1998. Plant cytogenetics. Silesian University, Katowice, Poland.
Fukui K., and Nakamya S. (1996). Plant chromosomes. Laboratory methods. CRC Press, Boca Raton.
Levin D. A. (2002) The role of chromosomal change in plant evolution. Oxford University Press, New York, USA.
Puertas M.J. & Naranjo T. (eds.) 2005. Plant cytogenetics. Karger, Basel.
Schwarzacher T., and Heslop-Harrison P. (2000) Practical in situ hybridization. 2nd ed. BIOS, Oxford UK.
Singh R.J. (2003) Plant cytogenetics. CRC Press, Boca Raton.
Stebbins G. L. (1971) Chromosomal evolution in higher plants. Edward Arnold, London.
Puertas M.J. & Naranjo T. (eds.) 2008. Plant cytogenetics. Karger, Basel.
Birchler J. & Pires C. (eds) 2010. Advances in Plant Cytogenetics. Karger, Basel.
Fukui K., and Nakamya S. (1996). Plant chromosomes. Laboratory methods. CRC Press, Boca Raton.
Levin D. A. (2002) The role of chromosomal change in plant evolution. Oxford University Press, New York, USA.
Puertas M.J. & Naranjo T. (eds.) 2005. Plant cytogenetics. Karger, Basel.
Schwarzacher T., and Heslop-Harrison P. (2000) Practical in situ hybridization. 2nd ed. BIOS, Oxford UK.
Singh R.J. (2003) Plant cytogenetics. CRC Press, Boca Raton.
Stebbins G. L. (1971) Chromosomal evolution in higher plants. Edward Arnold, London.
Puertas M.J. & Naranjo T. (eds.) 2008. Plant cytogenetics. Karger, Basel.
Birchler J. & Pires C. (eds) 2010. Advances in Plant Cytogenetics. Karger, Basel.
Zuordnung im Vorlesungsverzeichnis
MPF W-1, MEV W-6, MGE III-2
Letzte Änderung: Mo 07.09.2020 15:43