Universität Wien FIND

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Achtung! Das Lehrangebot ist noch nicht vollständig und wird bis Semesterbeginn laufend ergänzt.

300319 UE Plant Chromosome Structure and Evolution: theory and practice (2020W)

Chromosome analysis in flowering plants

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Prüfungsimmanente Lehrveranstaltung

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

max. 8 Teilnehmer*innen
Sprache: Englisch

Lehrende

Termine (iCal) - nächster Termin ist mit N markiert

Maximum of six students will be accepted this semester due to laboratory work (one week hands-on practical in the laboratory).

VB and first four lectures (13.10-17.11.2020) will be held digitally (in English).
Anwesenheitspflicht: 07.12.-11.12.2020, 09:00-17:00 Uhr, Labor 203/309 (Dept. für Botanik und Biodiversitätsforschung, Rennweg 14, 1030 Wien)

Students will work in pairs and specific schedule will be discussed during VB.
A short test (from material presented earlier; students will be advised which material will be required; presentations will be available on moodle) will be held during the practical part of the course (09.12.2020).

Dienstag 06.10. 11:00 - 12:00 Hybride Lehre
Dienstag 13.10. 11:00 - 13:00 Hybride Lehre
Dienstag 20.10. 11:00 - 13:00 Hybride Lehre
Dienstag 10.11. 11:00 - 13:00 Hybride Lehre
Dienstag 17.11. 11:00 - 13:00 Hybride Lehre

Information

Ziele, Inhalte und Methode der Lehrveranstaltung

The purpose of the course is two-fold. First, it offers an overview of plant cytogenetic techniques: from traditional chromosome counting to more recently established techniques of chromosome painting (in situ hybridization) and immunocytochemistry. Second, it presents detailed and up-to-date information about plant chromosome structure (centromere, telomere etc.) and main types of DNA sequences used for cytogenetic analyses. The newest methods developed recently to analyse genome composition and to characterize different types of repetitive DNA is plant genomes (high-throughput sequencing) will also be introduced. The course introduces also types and consequences of chromosome rearrangements including polyploidy, in the context of chromosome evolution. The use of cytogenetic data (obtained from both classical and molecular cytogenetic studies) in inferring plant taxa relationships and in evolutionary studies of different plant groups will be discussed. The course will give the up-to-date overview of the state-of-art in the analyses of plant chromosomes.
The practical part of the course will include chromosome preparation in selected groups of plants, as well as classical and molecular chromosome staining methods. The students learn to perform DNA:DNA in-situ hybridization (FISH), in particular to prepare and label the DNA probes (e.g., species-specific repetitive DNA, rDNAs, telomeric sequences), to detect of the hybridization sites and to analyse the obtained data (fluorescence microscopy). The students will acquire practical skills in chromosome handling and will learn to analyse and interpret chromosomal data in evolutionary context.

Art der Leistungskontrolle und erlaubte Hilfsmittel

Written test - theory from course material before practical starts 30% (15 points max)
Active participation in experiment planning and results analyses 40% (20 points max)
Written report summarizing results (in form of scientific manuscript) 30% (15 points max)

Min 50% for positive grade

KEY:
50-46 points - 1
45-39 points - 2
38-31 points - 3
30-25 points - 4
24.75 and below - 5 (below 50%)

Mindestanforderungen und Beurteilungsmaßstab

Aims
- To present and discuss in details the structure, types and function of chromosomes, and to emphasize their role in evolutionary processes.
- To present most relevant case studies.
- To introduce most important cytogenetic and genomic techniques, including state of the art next generation sequencing, and to discuss their application for evolutionary cytogenetics of plants.
- To introduce modern techniques used nowadays to study the structure and function of plant genomes and to emphasize the importance of such analyses as complementary approach for phylogenetic, evolutionary, or populational studies.
- To introduce methods for in situ-hybridization probe preparation and labelling, chromosome preparation and quality checks, and finally in situ hybridization (FISH).
Students will apply gathered knowledge to analyze and to interpret obtained results (light and fluorescent microscopy).

Prüfungsstoff

- Lectures
- Planning of the experiments; material pre-treatment and fixation; preparation of chromosome spreads using classical methods; documentation.
- Enzymatic chromosome preparations; DNA probe preparation and labelling (PCR labelling, nick translation); preparation of buffers and solutions for FISH.
- Checking of the quality of DNA probe labelling; FISH (fluorescent in situ hybridization); pretreatments, and hybridization.
- FISH: washing, detection and amplification.
- Analysis of FISH data (microscopy)

Literatur

Maluszynska J. (ed.) 1998. Plant cytogenetics. Silesian University, Katowice, Poland.
Fukui K., and Nakamya S. (1996). Plant chromosomes. Laboratory methods. CRC Press, Boca Raton.
Weiss-Schneeweiss H., and Schneeweiss G.M. 2012. Karyotype Evolution in Angiosperms. Springer
Levin D. A. (2002) The role of chromosomal change in plant evolution. Oxford University Press, New York, USA.
Puertas M.J. & Naranjo T. (eds.) 2005. Plant cytogenetics. Karger, Basel.
Schwarzacher T., and Heslop-Harrison P. (2000) Practical in situ hybridization. 2nd ed. BIOS, Oxford UK.
Singh R.J. (2003) Plant cytogenetics. CRC Press, Boca Raton.
Stebbins G. L. (1971) Chromosomal evolution in higher plants. Edward Arnold, London.
Puertas M.J. & Naranjo T. (eds.) 2008. Plant cytogenetics. Karger, Basel.
Birchler J. & Pires C. (eds) 2010. Advances in Plant Cytogenetics. Karger, Basel.
Plant Genome Diversity (book; 2 volumes; 2012 and 2013; Springer)

Zuordnung im Vorlesungsverzeichnis

MBO 5, MGE III-2, MES5

Letzte Änderung: Mo 05.10.2020 09:50