Universität Wien FIND
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301159 SE Protein Design and Evolution (2019W)

1.00 ECTS (1.00 SWS), SPL 30 - Biologie
Prüfungsimmanente Lehrveranstaltung

An/Abmeldung

Details

max. 25 Teilnehmer*innen
Sprache: Englisch

Lehrende

Termine

Vorlesende: Dr. Julia Shifman, Hebrew University, Jerusalem
Ort: Seminar raum 3. Stock, Dr. Bohr-Gasse
Zeitplan:
Monday, 11. Nov, 14.00-17.00
Wed, 13. Nov 14.00-17.00
Thurs, 14 Nov 14.00-16.00

In this course, I will survey topics in experimental and computational protein design and directed evolution. Protein design and directed evolution are two complimentary approaches that allow us to obtain proteins with a desired structure and/or function. While protein design is a top-down approach where proteins are modified rationally using computers or site-directed mutagenesis, directed evolution is a bottom-up design strategy that allows us to select proteins with improved properties. The beginning of the protein design field dates back to the early 1990's when the first simple alpha helical and beta sheet proteins were constructed from scratch. In the late nineties, computational methods have been introduced into the protein design field and have been since widely applied to design proteins de novo and to supplement existing proteins with a new function. I will discuss how protein design has been applied to engineer new enzymes, ligand sensors, nanomaterials, and therapeutic molecules. In the second part of the course the students will be introduced to various methods for directed evolution such as Phage display, Yeast surface display, and Ribosome display. I will discuss several applications of the directed evolution methodology to engineer better enzymes and to obtain inhibitors of various diseases-associated proteins starting with antibodies, various small protein domains, and natural protein effectors. Finally, I will give a few examples how computational design could be combined with experimental directed evolution to yield superior results in protein engineering.


Information

Ziele, Inhalte und Methode der Lehrveranstaltung

1) Introduction to protein engineering - basic principles and applications

2) Early studies in protein design, design of alpha helical and beta-sheet proteins

3) Methods for computational protein design

4) Computational design of novel structures

5) Computational protein design novel enzymes, inhibitors, and ligand receptors

6) Various strategies for directed evolution

7) Improving enzymes with directed evolution

8) Engineering binders and inhibitors with directed evolution methods

Art der Leistungskontrolle und erlaubte Hilfsmittel

Verpflichtende Anwesenheit. Die Beurteilung basiert auf folgenden Teilleistungen: Qualität und Aufbereitung der Seminarpräsentation, aktive Teilnahme an den Diskussionen der vorgestellten Publikationen, schriftliche Beiträge (die prozentuelle Aufteilung der Teilleistungen wird vom Kursleiter bekanntgegeben). Jede der Teilleistungen muss positiv sein

Mindestanforderungen und Beurteilungsmaßstab

Prüfungsstoff

Literatur


Zuordnung im Vorlesungsverzeichnis

PhD MB 1, M-WZB, MMB W-3

Letzte Änderung: Mo 04.11.2019 12:08