301234 VO Advanced Biochemical and Biophysical Methods (2019S)

Moodle

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Details

Sprache: Englisch

Prüfungstermine

Lehrende

Termine

VBC5 lecture hall A (Rm. 1.216)
Termine:
04.03. 09:00-10:30 Leonard From protein properties to purification
04.03. 10:45-12:15 Slade Protein expression and purification
05.03. 09:00-10:30 Stolt-Bergner Integral membrane proteins and protein complexes
05.03. 10:45-12:15 Zagrovic Math primer
06.03. 09:00-10:30 Sedivy Light Scattering and Absorption
06.03. 10:45-12:15 Sedivy Microcalorimetry
08.03. 09:00-10:30 Dong X-ray crystallography, SLS and SAXS (part 1)
08.03. 10:45-12:15 Dong X-ray crystallography, SLS and SAXS (part 2)
11.03. 09:00-10:30 Yudushkin Fluorescence spectroscopy
11.03. 10:45-12:15 Yudushkin Light microscopy
13.03. 09:00-10:30 Yudushkin Imaging methods for detection of molecular proximity
13.03. 10:45-12:15 Sedivy Applications of absorption, fluorescence and scattering
15.03. 09:00-10:30 Kontaxis NMR spectroscopy
15.03. 10:45-12:15 Reserved For last minute changes
18.03. 09:00-10:30 Reserved For last minute changes
18.03. 10:45-12:15 Reserved For last minute changes
20.03. 09:00-10:30 Heuser Electron microscopy
20.03. 10:45-12:15 Hartl Mass spectroscopy
22.03. 09:00-10:30 Reserved VBCF tour (upon demand)
22.03. 10:45-12:15 Reserved VBCF tour (upon demand)
25.03. 09:00-10:30 Sedivy Protein quality assessment and control
25.03. 10:45-12:15 Leonard Multi method approach to tackle real world examples
27.03. 09:00-10:30 Reserved VBCF tour (upon demand)/ exam
27.03. 10:45-12:15 Reserved VBCF tour (upon demand) / exam
29.03.- 09:00-10:30 Reserved VBCF tour (upon demand) / exam
29.03. 10:45-12:15 Reserved VBCF tour (upon demand) / exam

Information

Ziele, Inhalte und Methode der Lehrveranstaltung

This theoretical course will start with introductory lectures on the basic concepts in structural biology and cover modern approaches and fundamentals of the methods used in recombinant protein expression, purification, biophysical characterization and quality assessment of macromolecular samples as well as cell biophysics approaches. The covered topics include static and dynamic light scattering, fundamentals of X-ray crystallography, absorption and fluorescence spectroscopy techniques (CD, FRET, FLIM, FC(C)S, fluorescence anisotropy, etc), mass spectroscopy and imaging methods (light and electron microscopy).

Art der Leistungskontrolle und erlaubte Hilfsmittel

Mindestanforderungen und Beurteilungsmaßstab

Provide a comprehensive overview of strategies and methods for protein expression and purification; enable students to choose a better strategy for a given protein
Highlight the utility of different methods of protein characterization for understanding its structure and function using specific examples
Explain theoretical background and instrumentation used for protein characterization in amount sufficient for critical interpretation of original research results
Enable students to choose an adequate method for a specific question

Prüfungsstoff

Literatur

Zuordnung im Vorlesungsverzeichnis

PhD MB 3, MMB I-2, MMB II-2, M-WZB, MGE III-1,MMEI III, MMB W-2

Letzte Änderung: Mo 07.09.2020 15:44