Achtung! Das Lehrangebot ist noch nicht vollständig und wird bis Semesterbeginn laufend ergänzt.
301272 VO+UE Live imaging course (2023S)
Prüfungsimmanente Lehrveranstaltung
Labels
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Do 09.02.2023 08:00 bis Fr 10.03.2023 09:00
- Abmeldung bis Fr 10.03.2023 09:00
Details
max. 12 Teilnehmer*innen
Sprache: Englisch
Lehrende
Termine
VORBESPRECHUNG/PRELIMINARY MEETING:
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting Live Imaging Course SS23
Time: March 14th, 2023 11:00 AM Vienna
https://univienna.zoom.us/j/68370781722?pwd=NEZpNGtFYlhTM0syZFdHN1JzbUdWdz09
Meeting ID: 683 7078 1722
Passcode: 460209Attendance at the preliminary meeting is MANDATORY, since we expect more people to become registered for the course than seats are available!The course will be held JULY 4th-6th, 2023 - full day 9.30-17.30.
Location: Campus Vienna Biocenter; Building VBC5, Level E1.
The schedule will be available at Moodle: https://moodle.univie.ac.at/course/view.php?id=368631IMPORTANT INFORMATION (March 9th, 2023).
This year the course is heavily over-booked with 65 applicants for the 12 seats. Based on this situation, I am forced to apply the official SPL rules:
- Preference for students of the master studies UA 865, 830, 834, 877 – IRRESPECTIVE of the points bet.
- Students of the master studies UA 865, 830, 834, 877 will be ranked according to points bet
- Then all other studies, ranked by the points betMore details in the preliminary meeting on March 14th.
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Art der Leistungskontrolle und erlaubte Hilfsmittel
Continuous Assessment
Mindestanforderungen und Beurteilungsmaßstab
Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in extended practical hands-on-sessions using different live microscopy setups.
Prüfungsstoff
a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will be taught how to handle live imaging data in terms of processing and analysis
f. Students will learn and work in cooperative teams
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will be taught how to handle live imaging data in terms of processing and analysis
f. Students will learn and work in cooperative teams
Literatur
Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
www.fpbase.org
https://www.ibiology.org/talks/live-cell-imaging/
http://www.microscopyu.com/
https://www.olympus-lifescience.com/de/microscope-resource/
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/
https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
www.fpbase.org
https://www.ibiology.org/talks/live-cell-imaging/
http://www.microscopyu.com/
https://www.olympus-lifescience.com/de/microscope-resource/
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/
https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html
Zuordnung im Vorlesungsverzeichnis
MMEI III, MMB W-2, WZB, PhD (SPL 56), MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:
Letzte Änderung: Fr 02.06.2023 09:48
Five basic lectures:
- Lecture 1: Live Imaging Considerations and Techniques overview
- Lecture 2: Sample Preparation
- Lecture 3: Live Imaging Dyes and Fluorophores-basics and applications
- Lecture 4: Special techniques: FRAP, AIRY Scanning, Lattice SIM
- Lecture 5: Handling Live Imaging DataThree days of extensive hands-on sessions, planning and executing overnight and individual shorter time lapse experiments using various available microscope setups. The course includes half a day of computer hands-on to train handling of live imaging data (includes "file formats and data structure" / "necessary corrections" / "prepare video material for publications")