Universität Wien
Achtung! Das Lehrangebot ist noch nicht vollständig und wird bis Semesterbeginn laufend ergänzt.

301272 VO+UE Live imaging course - 2 SStd. (2025S)

2.00 ECTS (2.00 SWS), SPL 30 - Biologie
Prüfungsimmanente Lehrveranstaltung

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

max. 12 Teilnehmer*innen
Sprache: Englisch

Lehrende

Termine

COURSE LANGUAGE IS ENGLISH!!

VORBESPRECHUNG/PRELIMINARY MEETING:
Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting Live Imaging Course
Time: Mar 18, 2025 11:00 AM Vienna
Join Zoom Meeting
https://univienna.zoom.us/j/69731534478?pwd=PeonLl9jUUgrzdTq84QtABJLfN0krw.1
Meeting ID: 697 3153 4478
Passcode: 522537

Attendance at the preliminary meeting is MANDATORY, since we expect more people to become registered for the course than seats are available!

The course will be held JULY, 8th-10th, 2025 - full day 9.45-17.30.
Location: Campus Vienna Biocenter; Building VBC5, Level E1.
The schedule will be available at Moodle: https://moodle.univie.ac.at/course/view.php?id=441148

IMPORTANT INFORMATION
The course may be heavily over-booked for the 12 available seats.
Based on this situation, I will apply the official SPL rules:
- For the first 10 seats, preference will be given to students of the master studies UA 865, 830, 834, 877.
- Students of the master studies UA 865, 830, 834, 877 will be ranked according to points bet.
- The last 2 seats will be quoted to studies with a natural sciences background. Students of these studies will then also become ranked by their points bet.

More details in the preliminary meeting on March 18th, 2025.
A list of the successful participants (according to the rules described above) will be announced at the preliminary meeting

All others will be put on the waiting list. If later seats become vacant, the following rules will be applied
Attendees of the preliminary meeting will be ranked up (according to their points bet).
If seats become vacant, the waiting list will be grouped in persons of 5.
The first 5 people (= group1) on the waiting list will be informed - the seats will be assigned then on a "first come first served" basis.
Then the next 5 people on the list (group 2) are considered, and so on and so on.


Information

Ziele, Inhalte und Methode der Lehrveranstaltung

The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using modern light microscopic technologies, like confocal, spinning disc, fluorescence widefield, superresolution and high-content imaging microscopy. A session of data handling complements the microscope-related data acquisition.
Five basic lectures:
- Lecture 1: Live Imaging Considerations and Techniques overview
- Lecture 2: Sample Preparation
- Lecture 3: Live Imaging Dyes and Fluorophores-basics and applications
- Lecture 4: Special techniques: FRAP, AIRY Scanning, Lattice SIM
- Lecture 5/HANDS-ON: Handling Live Imaging Data

Three days of extensive hands-on sessions, planning and executing overnight and individual shorter time lapse experiments using various available microscope setups. The course includes half a day of computer hands-on to train handling of live imaging data (includes "file formats and data structure" / "necessary corrections" / "prepare video material for publications")

Art der Leistungskontrolle und erlaubte Hilfsmittel

Continuous Assessment

Mindestanforderungen und Beurteilungsmaßstab

Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in extended practical hands-on-sessions using different live microscopy setups. One part of the course is dedicated to data handling from live imaging experiments.

Prüfungsstoff

a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will be taught how to handle live imaging data in terms of processing and analysis
f. Students will learn and work in cooperative teams

Literatur

Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
www.fpbase.org
https://www.ibiology.org/talks/live-cell-imaging/
http://www.microscopyu.com/
https://www.olympus-lifescience.com/de/microscope-resource/
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/
https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html

Zuordnung im Vorlesungsverzeichnis

MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:, PhD (SPL 56)

Letzte Änderung: Fr 17.01.2025 15:06