Universität Wien

301273 VO+UE Live imaging course (2024W)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Prüfungsimmanente Lehrveranstaltung

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

max. 12 Teilnehmer*innen
Sprache: Englisch

Lehrende

Termine

COURSE LANGUAGE IS ENGLISH!!

VORBESPRECHUNG/PRELIMINARY MEETING:

Josef Gotzmann is inviting you to a scheduled Zoom meeting.
Topic: Preliminary Meeting LiveImagingCourse WS2024-25
Time: Oct 7, 2024 11:00 AM Vienna
Join Zoom Meeting at https://univienna.zoom.us/j/68118830211?pwd=bQkwNe3kRiudkt4xehNmqikB9plw6a.1
Meeting ID: 681 1883 0211
Passcode: 995479

Attendance at the preliminary meeting is MANDATORY, since we expect more people to become registered for the course than seats are available!

The course will be held FEBRUARY 4th-6th, 2025 - full day 9.45-17.30.
Location: Campus Vienna Biocenter; Building VBC5, Level E1.
The schedule will be available at Moodle: https://moodle.univie.ac.at/course/edit.php?id=433242

IMPORTANT INFORMATION
The course may be heavily over-booked for the 12 available seats.
Based on this situation, I will apply the official SPL rules:
- For the first 10 seats, preference will be given to students of the master studies UA 865, 830, 834, 877.
- Students of the master studies UA 865, 830, 834, 877 will be ranked according to points bet.
- The last 2 seats will be quoted to studies with a natural sciences background. Students of these studies will then also become ranked by their points bet.

More details in the preliminary meeting on October, 7th.
A list of the successful participants (according to the rules described above) will be announced at the preliminary meeting

All others will be put on the waiting list. If later seats become vacant, the following rules will be applied
Attendees of the preliminary meeting will be ranked up (according to their points bet).
If seats become vacant, the waiting list will be grouped in persons of 5.
The first 5 people (= group1) on the waiting list will be informed - the seats will be assigned then on a "first come first served" basis.
Then the next 5 people on the list (group 2) are considered, and so on and so on.


Information

Ziele, Inhalte und Methode der Lehrveranstaltung

Our "Live Imaging Course" will for the first time shine in an extended format: The course is planned for three days and 12 attendees will be allowed as we stockpiled the teaching personnel capacity. New will be a live superresolution microscopy experience , as well as a session dedicated on handling and processing data from live imaging experiments.
The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using advanced light microscopic technologies, like confocal, spinning disc, fluorescence widefield, high-content and even superresolution imaging microscopy.
In total 5 lectures will be held on the following topics:
- Live Imaging Considerations
- Sample preparation
- Dyes and Fluorophores
- Specialized Techniques (FRAP, AIRY SCAN, LATTICE SIM)
- Handling Live Imaging Data

Extensive hands-on planning of overnight and individual shorter time lapse experiments using various available microscope setups.
An afternoon is dedicated to give expert advice on how to handle live imaging data - this will include selected examples as well as insight into advanced processing pipelines, including aspects of Artificial Intelligence.

Art der Leistungskontrolle und erlaubte Hilfsmittel

Continuous Assessment

Mindestanforderungen und Beurteilungsmaßstab

Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in extended practical hands-on-sessions using different live microscopy setups. Eminently important is to prepare students for handling the data with respect to size, processing and analyses.

Prüfungsstoff

a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will learn and work in cooperative teams
f. Students will get a comprehensive picture by being introduced into data management

Literatur

Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009www.fpbase.orghttps://www.ibiology.org/talks/live-cell-imaging/http://www.microscopyu.com/https://www.olympus-lifescience.com/de/microscope-resource/http://zeiss-campus.magnet.fsu.edu/index.htmlhttp://www.leica-microsystems.com/science-lab/https://www.rms.org.uk/resources-downloads/online-microscopy-talks.html

Zuordnung im Vorlesungsverzeichnis

MMEI III, PhD (SPL 56), MMB III-1a:, MMB III-2a:, MMB III-3a:, MMB III-4a:

Letzte Änderung: Fr 30.08.2024 13:06