Universität Wien

301417 VO Methods in Molecular Biology (2022W)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
GEMISCHT

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

Sprache: Englisch

Prüfungstermine

Lehrende

Termine (iCal) - nächster Termin ist mit N markiert

COVID-19 Information:
According to the actual COVID-19 rules of the University of Vienna:
- Don't come to the University if you are sick!
- Don't attend the lectures/exams, if you have been positively tested for COVID-19. Attendance via u:stream will be possible for this lecture series.
- Please wear an FFP2-mask, while being in the lecture hall. This is not mandatory, but we hauntingly request you to behave empathically by wearing an FFP2 mask.
Best,
Josef Gotzmann (Sept 28, 2022)
***
To date (June 24, 2022) ALL lectures will be held with PHYSICAL PRESENCE! Any changes due to revised COVID regulations will become announced in time. ALL lectures are held at the new "Biology Building" - Lecture hall 2.
More information will be uploaded to Moodle: https://moodle.univie.ac.at/course/view.php?id=328483

All lectures will be available via u:stream (link at Moodle:
https://moodle.univie.ac.at/mod/streamlti/view.php?id=14526015

Schedule (Sept28, 2022; changes still possible):
1. Thursday, October 6th , 11.30-13.00: DNA Repair and Remodeling Methods; includes Noble 2015 topic “DNA Repair”; Dea Slade
2. Friday, October 7th , 11.30-13.00: Next Generation Sequencing 1: Theory and Technologies; Andreas Sommer
3. Tuesday, October 11th , 15.00-16.30: Next Generation Sequencing 2: Mehtods and Applications; Andreas Sommer
4. Wednesday, October 12th , 15.00-16.30: Genome editing with a focus on CRISPR Methodology (Noble 2020 Lecture); Krzysztof Chylinski
5. Wednesday, October 19th , 15.00-16.30: Mass Spectrometry and Advanced Proteomics; Christopher Gerner
6. Thursday, October 20th , 11.30-13.00: Membrane Biology and Lipid Biochemistry Methods, including Screening / Lipidomics; Robert Ahrends
7. Monday, October 24th , 15.00-16.30: Light Microscopy Techniques-Applicative Potential; Josef Gotzmann
8. Tuesday, October 25th , 15.00-16.30: Super-Resolution Microscopy (Noble 2014 topic); Thomas Peterbauer
9. Monday, November 7th , 15.00-16.30: Fluorescence microscopy methods in studies of cellular physiology; includes Noble 2019 topic “Methods to study oxygen sensing”; Ivan Yudushkin
10. Tuesday, November 8th , 15.00-16.30: Organoids and Developmental Biology Methods; Elly Tanaka
11. Wednesday, November 9th , 15.00-16.30: Structural Biology & X-ray Crystallography; includes Noble 2017 topic “Cryo-EM”; Gang Dong
12. Tuesday, November 15th , 15.00-16.30: Methods in Plant Biology; Andreas Bachmair
13. Wednesday, November 16th , 15.00-16.30: Biochemical Reconstitution Assays; Martin Loose
14. Friday, November 18th , 15.00-16.30: Stem Cell Biology and Genetic Screening; Martin Leeb

As lecture 11 (planned Nov 9) had to be canceled, the NEW DATE for this lecture (Structural Biology... by Gang Dong) is now TUESDAY, NOV 15th, 16h45-18h15 in HS1 at the UBB building.

Josef Gotzmann (Nov 9)

  • Donnerstag 06.10. 11:30 - 13:00 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Freitag 07.10. 11:30 - 13:00 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Dienstag 11.10. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Mittwoch 12.10. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Mittwoch 19.10. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Donnerstag 20.10. 11:30 - 13:00 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Montag 24.10. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Dienstag 25.10. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Montag 07.11. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Dienstag 08.11. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Mittwoch 09.11. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Dienstag 15.11. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Dienstag 15.11. 16:45 - 18:15 Hörsaal 1, Biologie Djerassiplatz 1, 0.037, Ebene 0
  • Mittwoch 16.11. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0
  • Freitag 18.11. 15:00 - 16:30 Hörsaal 2, Biologie Djerassiplatz 1, 0.033, Ebene 0

Information

Ziele, Inhalte und Methode der Lehrveranstaltung

Die Vorlesung behandelt neue und innovative Methoden und spezifische Anwendung in der molekularbiologischen Forschung, und ist als weiterführende Lehrveranstaltung konzipiert, die auf den im Bachelorstudium erhaltenen theoretischen und praktischen Kenntnissen aufbaut. Das heißt grundlegende Kenntnisse von Standardtechniken in der Zellbiologie (z.B.: Zellkultur, Transfektion, PCR, etc) werden vorausgesetzt.Die Inhalte der Vorlesungsreihe werden jährlich adaptiert, um neue Entwicklungen einzubauen. Wesentliche, in der Vorlesung enthaltenen Bereiche, sind: Moderne Anwendungen in Pflanzenbiologie, in der Lichtmikroskopie, Rekonstitutionsanalysen, entwicklungsbiologische Methoden, Genomeditierungsmethoden, Protein-Protein Wechselwirkungen, DNA Reparaturanalysen, Next-Generation Sequencing, strukturbiologische Methoden und dedizierte Verfahren zur Proteinanalyse.
Wir planen alle Vorlesungen per u:Stream aufzuzeichnen.

Art der Leistungskontrolle und erlaubte Hilfsmittel

Schriftliche Abschlussprüfung.Zum jetzigen Zeitpunkt planen wir die Prüfungen VOR ORT (mit Präsenz) abzuhalten. Jegliche Änderungen durch unabsehbare COVID-19 Maßnahmen werden zeitgerecht bekannt gegeben. Wir planen alle 4 Prüfungstermine aus Gründen der Fairness im selben Format abzuhalten.

Mindestanforderungen und Beurteilungsmaßstab

It is the principle aim of this lecture series to give people advanced in their study an overview on selected techniques and technologies, which are beyond standard textbook knowledge. A series of declared experts and specialists, who also work with these techniques in everyday lab-life, will allow students to get a deeper insight into how profound scientific research is conducted on a methodological level.
@Exam: 6 questions (each from a different topic) - each question 10 points maximum.
The exam questions style is completely free to every lecturer: can contain several sub-questions, can be open questions, multiple choice, single choice or Pick-N-style and also a mixture of question types.
Evaluation key: 1 (Sehr Gut – A): 55,01-60,00
2 (Gut - B): 48,01-55,00
3 (Befriedigend - C): 39,01-48,00
4 (Genügend - D): 30,00-39,00
5 (Nicht Genügend – E / Failed): 00,00-29,99

Prüfungsstoff

The slide contents of the individual lectures is the basis for the exam questions. Any added oral/written comments from the individual lecturers will also be included. PLEASE NOTE THAT IF Exams MUST BE IN "OPEN BOOK" FORM, THE QUESTIONS MIGHT BE MORE GENERAL, AS STUDENTS MAY BE ALLOWED TO USE DIFFERENT SOURCES OF BACKGROUND INFORMATION!

Literatur

shall and will be announced by each lecturer

Zuordnung im Vorlesungsverzeichnis

MMB I., MMB III-2, MMB IV-2, MMB IV-3, MMB W-2, MMEI III

Letzte Änderung: Mo 23.01.2023 14:09