301450 VO Makromolekulare Kristallographie (2020S)

The following topics will be covered the major topic of macromolecular crystallography and will provide students with a comprehensive overview of all the steps required to generate a three-dimensional structural information on biological macromolecules:
* sources of X-rays
* crystallisation techniques
* symmetry (point groups, space groups), unit cell, Bravais lattice
* scattering of X-rays, atomic form factor, structure factor
* waves, diffraction of molecules and crystals, Fourier transform; electron density
* set-up of MX diffraction experiment and detectors
* methods for solution of phase problem: molecular replacement, multiple isomorphous replacement, multi-wavelength anomalous dispersion
* density modification techniques
* structure refinement, structure validation and analysis