301843 UE Practical Course in Molecular and Cellular Immunobiology (2023S)
Prüfungsimmanente Lehrveranstaltung
Labels
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Do 09.02.2023 08:00 bis Di 30.05.2023 09:00
- Abmeldung bis Di 30.05.2023 09:00
Details
max. 18 Teilnehmer*innen
Sprache: Englisch
Lehrende
Termine (iCal) - nächster Termin ist mit N markiert
Preliminary meeting/Vorbesprechung: Thursday 1st of June 2023 at 11:00.
Attending the preliminary meeting is critical for securing a place in the course. If you cannot attend, please make sure to send a representative in your place. The preliminary meeting will take place online via Zoom, a link for which will be emailed to all registered students after the registration closes on May 30th at 15:00.
Course: 19th of June - 7th of July 2023 9.00 - 17.00h
Exam Thursday 13th of July 13:00-14:30 - SR 1/6.501
- Freitag 16.06. 13:00 - 17:00 BZB/Seminarraum 3, 6.Ebene 6.105, Dr.-Bohrgasse 9, 1030 Wien (Vorbesprechung)
- Montag 19.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Dienstag 20.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Mittwoch 21.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Donnerstag 22.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Freitag 23.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Montag 26.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Dienstag 27.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Mittwoch 28.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Donnerstag 29.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Freitag 30.06. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Montag 03.07. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Dienstag 04.07. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Mittwoch 05.07. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Donnerstag 06.07. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Freitag 07.07. 08:55 - 17:00 BZB-Übungsraum 2D, 6.Ebene 6.526, Dr.-Bohrgasse 9, 1030 Wien
- Donnerstag 13.07. 13:00 - 14:30 BZB/Seminarraum 1/2, 6.Ebene 6.501/6.504, Dr.-Bohr-Gasse 9, 1030 Wien
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Art der Leistungskontrolle und erlaubte Hilfsmittel
Students are assessed on the following criteria:1) Performance during the course (practical skills, knowledge and reasoning during black-board discussion, initiative and participation).2) Written exam; tests for knowledge, ability to apply knowledge, and perform scientific calculations relevant to the course material.3) Written report in scientific manuscript format; includes quality of the data, and experimental outcome.During the practical days of the course, use of the course manual and calculators is allowed. During the written exam, only the use of a standard calculator is allowed.
Mindestanforderungen und Beurteilungsmaßstab
a. Minimum requirements: Compulsory attendance (attendance during the whole course is a minimum requirement); the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam. All students must have familiarized themselves with the course manual before start of the practical work.b. Assessment criteria:Example for a course with continuous assessment: You can achieve 100 points1) Performance during the course: 45 points.2) Written exam: 30 points.3) Written report, and data quality/ experimental outcome: 25 pointsGrading example: 1 (excellent) 100 90 points 2 (good) 89 81 points 3 (satisfactory) 80 71 points 4 (sufficient) 70 - 61 points 5 (insufficient) 60 0 points
Prüfungsstoff
- All information in the course manual - All information provided in non-written form during daily ‘blackboard’ teaching during the course; includes discussions of data interpretation and expected outcomes of experiments. - Scientific calculations relevant to the course material. - All information from introductory lectures
Literatur
The provided course manuals.
Zuordnung im Vorlesungsverzeichnis
MMEI I, Biologische Chemie: BIO II-2, MMB III-2a:, MMB III-3a:, MMB III-4a:
Letzte Änderung: Mi 12.07.2023 13:47
a. Aims: Students should learn immunological techniques, e.g. working with cell culture (sterile handling of cell lines, transfection of cells, isolation and differentiation of primary cells from bone marrow and spleen), antiviral assays, fluorescence microscopy, luciferase assays, FACS, purification of antibodies, immune precipitation, Western blot. Acquiring knowledge and experimental skills concerning the innate immune system, and functional assays on genetically modified mammalian cells.
b. methods and contents: In this practical course (hands-on) we will use CRISPR-Cas9 technology to make knock-outs of key genes of the innate immune system in human cells. The generated cells will be functionally tested using reporter assays.