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301843 UE Practical Course in Molecular and Cellular Immunobiology (2025S)
Prüfungsimmanente Lehrveranstaltung
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max. 18 Teilnehmer*innen
Sprache: Englisch
Lehrende
Termine
Preliminary meeting/Vorbesprechung: Wednesday, May 7, 2025, 9:00 – 10:00, 6th floor seminar room 1 (room 6.501). Participation in the preliminary discussion is a prerequisite for admission to the course. A representative may also attend if it is not possible to attend in person.
Introductory lectures: Thursday, June 5, 2025, 13:00-17:00, Seminar Room Floor 3 (room 3.202), ZMB, Max Perutz Labs, Dr. Bohr-Gasse 9
Course: Tuesday, June 10 – Friday, June 27, 2025, 9.00 - 17.00h, Übungsräume/course rooms 6th floor, Max Perutz Labs, BZB, Dr.Bohrgasse 9, 1030 Wien
Exam: Thursday July 3, 2025, 12:30 - 14:00, 6th floor seminar room 1 (room 6.501), Max Perutz Labs, BZB, Dr.Bohrgasse 9, 1030 Wien
1. Attendance at the preliminary meeting (‘Vorbesprechung’);
2. Preference is given to students in the master’s curriculum A 066 830 'Molecular Microbiology, Microbial Ecology and Immunobiology', and to students who completed the courses 301844 SE Advanced Techniques in Molecular and Cellular Immunobiology and 301008 PS Proseminar in Immunobiology.
3. Additional requirements for students who have not completed their bachelor's degree in the bachelor's program Biology at the University of Vienna in the areas of BMG or BMB: External students requested to fulfil specific admission requirements (e.g. practical courses, UE) for the admission to the Master´s program need to have to completed these requirements before the admission to the practical course.
4. Students from other disciplines must have completed an introductory course in immunology (e.g., Immunology and Cellular Microbiology A).
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
Art der Leistungskontrolle und erlaubte Hilfsmittel
Students are assessed on the following criteria:1) Performance during the course (practical skills, knowledge and reasoning during black-board discussion, initiative and participation).2) Written exam; tests for knowledge, ability to apply knowledge, and perform scientific calculations relevant to the course material.3) Written report in scientific manuscript format; includes quality of the data, and experimental outcome.During the practical days of the course, use of the course manual and calculators is allowed. During the written exam, only the use of a standard calculator is allowed.
Mindestanforderungen und Beurteilungsmaßstab
a. Minimum requirements: Compulsory attendance (attendance during the whole course is a minimum requirement); the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam. All students must have familiarized themselves with the course manual before start of the practical work.b. Assessment criteria:Example for a course with continuous assessment: You can achieve 100 points1) Performance during the course: 45 points.2) Written exam: 30 points.3) Written report, and data quality/ experimental outcome: 25 pointsGrading example: 1 (excellent) 100 90 points 2 (good) 89 81 points 3 (satisfactory) 80 71 points 4 (sufficient) 70 - 61 points 5 (insufficient) 60 0 points
Prüfungsstoff
- All information in the course manual - All information provided in non-written form during daily ‘blackboard’ teaching during the course; includes discussions of data interpretation and expected outcomes of experiments. - Scientific calculations relevant to the course material. - All information from introductory lectures
Literatur
The provided course manuals.
Zuordnung im Vorlesungsverzeichnis
MMEI I
Letzte Änderung: Fr 10.01.2025 00:02
a. Aims: Students should learn immunological techniques, e.g. working with cell culture (sterile handling of cell lines, transfection of cells, isolation and differentiation of primary cells from bone marrow and spleen), antiviral assays, fluorescence microscopy, luciferase assays, FACS, purification of antibodies, immune precipitation, Western blot. Acquiring knowledge and experimental skills concerning the innate immune system, and functional assays on genetically modified mammalian cells.
b. methods and contents: In this practical course (hands-on) we will use CRISPR-Cas9 technology to make knock-outs of key genes of the innate immune system in human cells. The generated cells will be functionally tested using reporter assays.