Universität Wien
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580012 VU Introduction to method development and validation for LCMS/MS qualitative and quantitative analysis (2021W)

1.00 ECTS (1.00 SWS), SPL 58 - Doktoratsstudium Pharmazie, Ernährungswissenschaften und Sportwissensch
Prüfungsimmanente Lehrveranstaltung
Moodle

An/Abmeldung

Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").

Details

Sprache: Deutsch

Lehrende

Termine

Location: Seminar room 2D404 - UZ2
-Wearing a FFP2 face mask is mandatory!
- 2.5G Regulation : On-site participation only when tested, vaccinated or if you have recovered from an infection.

15.10.2021: 09.15h - 10.45h (2 x 45 min) - > Cancelled
22.10.2021: 09.15h - 10.45h (2 x 45 min)
05.11.2021: 09.15h - 10.45h (2 x 45 min)
LAB Day I: Dates to be arranged based on students needs and schedule on first lecture (5 x 45 min )
LAB Day II: Dates to be arranged based on students needs and schedule on first lecture (5 x 45 min )

Information

Ziele, Inhalte und Methode der Lehrveranstaltung

This course endows an introduction to the crucial notions and approaches in the method development and validation for LC–MSMS qualitative and quantitative analysis. Prominence will be shed on development of efficient, straight forward and universal methods used in common LC-MS Analysis. The aim of the course is to coach the students how to develop efficient LC methods, couple them to MS methods, understand, optimize and tune ESI source parameters. The course addresses various problems confronted when perusing studies/carrier in the field of MS oriented analytical chemistry. The course includes case studies in the fields of LC-MS analysis and small molecules analyses (OMICS).

Art der Leistungskontrolle und erlaubte Hilfsmittel

• Understand, optimize and tune LC parameters
• Understand, optimize and tune ESI source parameters
• Understand, optimize and tune MS experiments parameters
• Develop an outline of the key procedures and tools that are used in MS data analysis
• Be capable of developing at least: one untargeted experiment (in omics oriented fashion) and one targeted experiment (in 3Q absolute quantification fashion)

Mindestanforderungen und Beurteilungsmaßstab

• 322063 VU Analytical Chemistry in Natural Product Research - M14 “Recommended”
• Laptop

Prüfungsstoff

Grade will be given based on three aspects:
1. Final evaluation based on self-developed methods
2. Lab assignments

Literatur

Zuordnung im Vorlesungsverzeichnis

Letzte Änderung: Mi 13.10.2021 16:30