580012 VU Introduction to method development and validation for LCMS/MS qualitative and quantitative analysis (2023W)
1.00 ECTS (1.00 SWS), SPL 58 - Doktoratsstudium Pharmazie, Ernährungswissenschaften und Sportwissensch
Prüfungsimmanente Lehrveranstaltung
Labels
An/Abmeldung
Hinweis: Ihr Anmeldezeitpunkt innerhalb der Frist hat keine Auswirkungen auf die Platzvergabe (kein "first come, first served").
- Anmeldung von Fr 22.09.2023 12:00 bis Mo 13.11.2023 12:00
- Abmeldung bis Mo 13.11.2023 12:00
Details
Sprache: Deutsch
Lehrende
Termine
13.11.2023--> 09.00h-11.15h
14.11.2023--> 09.00h-11.15h
15.11.2023--> 09.00h-11.15h
16.11.2023--> 09.00h-11.15h
17.11.2023--> 09.00h-11.15h
Information
Ziele, Inhalte und Methode der Lehrveranstaltung
This course endows an introduction to the crucial notions and approaches in the method development and validation for LC–MSMS qualitative and quantitative analysis. Prominence will be shed on development of efficient, straight forward and universal methods used in common LC-MS Analysis. The aim of the course is to coach the students how to develop efficient LC methods, couple them to MS methods, understand, optimize and tune ESI source parameters. The course addresses various problems confronted when perusing studies/carrier in the field of MS oriented analytical chemistry. The course includes case studies in the fields of LC-MS analysis and small molecules analyses (OMICS).
Art der Leistungskontrolle und erlaubte Hilfsmittel
• Understand, optimize and tune LC parameters
• Understand, optimize and tune ESI source parameters
• Understand, optimize and tune MS experiments parameters
• Develop an outline of the key procedures and tools that are used in MS data analysis
• Be capable of developing at least: one untargeted experiment (in omics oriented fashion) and one targeted experiment (in 3Q absolute quantification fashion)
• Understand, optimize and tune ESI source parameters
• Understand, optimize and tune MS experiments parameters
• Develop an outline of the key procedures and tools that are used in MS data analysis
• Be capable of developing at least: one untargeted experiment (in omics oriented fashion) and one targeted experiment (in 3Q absolute quantification fashion)
Mindestanforderungen und Beurteilungsmaßstab
•Windows Laptop
Prüfungsstoff
Grade will be given based on three aspects:
1. Final evaluation based on self-developed methods
2. Lab assignments
1. Final evaluation based on self-developed methods
2. Lab assignments
Literatur
Zuordnung im Vorlesungsverzeichnis
Letzte Änderung: Fr 22.09.2023 10:28