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270025 VO Semisynthesis of Proteins (2017S)
Methods for site-specific protein modifications by chemical and molecular biolobigy approaches
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Fr 9:15-10:45 im HS IV
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Language: German
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The lecture starts on Friday, March 3, 9:15 am, lecture hall IV.
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BC-3, CHE II-3, CHE II-4.
Last modified: Mo 07.09.2020 15:41
http://www.univie.ac.at/biologischechemie1/semisynthese/Auxotrophie-Stämme: globaler Einbau unnatürlicher Aminosäuren, SelenomethioninIn vitro Suppression: Amber-Codon, chemisch beladene Suppressor-tRNAs, OrtsspezifitätIn vivo Suppression: Orthogonales tRNA/ AaRS-Paar, ProteinevolutionChemische Modifikation von Proteinen: Biokonjugation der proteinogenen Aminosäuren (Lys, Cys),
Oxidation N-Terminus, PLP-vermittelte Ligation, Cross-LinkerFluoreszierende Proteine: GFP und weitere BeispieleChemoselektive Ligationsreaktionen: Biokonjugation artifizieller Gruppen, Oxim-Ligation, Cu(I)-katalysierte 1,3-dipolare Cycloaddition, Staudinger-Ligation, Modifikation an lebenden ZellenChemische Peptid-/ Proteinligation: Peptidfestphasensynthese, Sekundärstrukturbrecher, Peptidsegmentkopplung, Native Chemische Ligation, Cys-freie Ligation, Auxiliare, Inteine, Expressed Protein LigationEnzymatische Peptidligation: Reverse Proteolyse, Sortase, gespaltene Inteine