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270025 UE Opt. Pract. Exercises: Proteinanalytics (2018W)

3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first serve).

Details

max. 12 participants
Language: German

Lecturers

Classes (iCal) - next class is marked with N

1. Kurs: 01.-05.10.2018
2. Kurs: 08.-12.10.2018
ANWESENHEITSPFLICHT FÜR DIE TEILNEHMER BEIDER KURSE AM 1. 10. um 10:00 im SR3 !
Theorieteil für BEIDE KURSE Mo. 01.10. 10-12 Uhr SEM 3, DO. 04.10. 13-15 Uhr SEM 3
Zeitgleich findet am 01.10.2018 die Vorbesprechung für das HPLC Praktikum statt.
Es gilt Anwesenheitspflicht. Teilnehmer auf der Warteliste können bei Nicht Anwesenheit vorrücken.

Monday 01.10. 10:00 - 12:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
Thursday 04.10. 13:00 - 15:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1

Information

Aims, contents and method of the course

The proteome of a complex sample shall be analyzed. Therefore, first, the complexity of the sample will be reduced using appropriate separation techniques. After that, an analysis of the sample, applyig mass spectrometry, will be performed. In the course of this practical work, different methods for protein analysis will be presented and practically applied.

Assessment and permitted materials

Performance is monitored during the practical course (interest, cooperation), and furthermore determined on the basis of the quality of the delivered protocol and the acquired knowledge (determined in the closing meeting).

Minimum requirements and assessment criteria

Acquisition of the most important knowledge and skills necessary to work with complex biological samples and allow a subsequent proteome analysis using LC-MS/MS.

Examination topics

Background knowledge on the conducted experiments: preparation of biological samples (blood samples), isolation of different blood constitutents (blood plasma and white blood cells), subcellular fractionation (separation of cytoplasmic and nuclear proteins), protein separation techniques using 2D- and 1D-gel electrophoresis, digestion of proteins into petides, identification of proteins using LC-MS/MS analyses.

Reading list

Additional literature is not required.

Association in the course directory

AN-1, AN-2, BC-1, CHE II-1

Last modified: Mo 07.09.2020 15:41