270025 UE Opt. Pract. Exercises: Proteinanalytics (2019W)
3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Continuous assessment of course work
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Su 01.09.2019 08:00 to We 25.09.2019 23:59
- Deregistration possible until We 25.09.2019 23:59
max. 12 participants
Classes (iCal) - next class is marked with N
Kurs 1: 1.10.-7.10.2019
Kurs 2: 7.10.-11.10.2019
Tuesday 01.10. 10:00 - 12:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1 (Kickoff Class)
Thursday 03.10. 13:00 - 15:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
Aims, contents and method of the course
The proteome of a complex sample shall be analyzed. Therefore, first, the complexity of the sample will be reduced using appropriate separation techniques. After that, an analysis of the sample, applyig mass spectrometry, will be performed. In the course of this practical work, different methods for protein analysis will be presented and practically applied.
Assessment and permitted materials
Performance is monitored during the practical course (interest, cooperation), and furthermore determined on the basis of the quality of the delivered protocol and the acquired knowledge (determined in the closing meeting).
Minimum requirements and assessment criteria
Acquisition of the most important knowledge and skills necessary to work with complex biological samples and allow a subsequent proteome analysis using LC-MS/MS.
Background knowledge on the conducted experiments: preparation of biological samples (blood samples), isolation of different blood constitutents (blood plasma and white blood cells), subcellular fractionation (separation of cytoplasmic and nuclear proteins), protein separation techniques using 2D- and 1D-gel electrophoresis, digestion of proteins into petides, identification of proteins using LC-MS/MS analyses.
Additional literature is not required.
Association in the course directory
AN-1, AN-2, BC-1, CHE II-1
Last modified: Mo 07.09.2020 15:21